ATP适体接枝Fe3O4纳米粒子的制备及化学发光酶检测应用
Nanoparticles Grafted by ATP Aptamer: Preparation and Application in Chemiluminescence Enzyme Analysis
作者单位E-mail
严喜鸾 南昌大学资源环境与化工学院, 南昌 330031  
陈馨 南昌大学资源环境与化工学院, 南昌 330031  
高文菊 南昌大学资源环境与化工学院, 南昌 330031  
魏婷婷 南昌大学资源环境与化工学院, 南昌 330031  
林月敏 南昌大学资源环境与化工学院, 南昌 330031  
艾凡荣 南昌大学机电工程学院, 南昌 330031
南昌大学转化医学研究院, 南昌 330031 
afr3755875@126.com 
摘要: 制备了油酸修饰的Fe3O4纳米粒子,利用盐酸多巴胺对其表面进行氨基化改性,制得水分散性良好的Fe3O4纳米粒子,用X射线衍射、透射电镜、傅里叶变换红外光谱仪、振动样品磁强计和紫外-可见吸收光谱进行表征。随后,将氨基修饰的三磷酸腺苷(ATP)适体接枝到Fe3O4纳米粒子上,结合荧光素酶化学发光法进行ATP的定量检测,并应用于市售酸奶中乳酸菌ATP含量的检测,其灵敏度高、重现性好。各项实验结果表明所制备的Fe3O4纳米粒子是一种分散性好、易分离的载体,其粒径均一、稳定、磁性强、与适体结合性能好,拓展了Fe3O4纳米粒子在分析检测领域的应用。
关键词: Fe3O4  纳米粒子  氨基功能化  适体  化学发光检测
基金项目: 国家自然科学基金(No.81102289,51102131,31660491)和江西省自然科学基金(No.20132BAB205106,20142BAB216033)资助项目。
Abstract: Oleic acid-modified Fe3O4 nanoparticles was prepared and dopamine hydrochloride was chosen to amino-modify these nanoparticles. The Fe3O4 nanoparticles have good water-dispersible, and were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) spectroscopy, vibrating sample magnetometer and ultravioletvisible spectroscopy (UV-Vis) spectra. Then, amino-modified adenosine triphosphate (ATP) aptamer was grafted onto Fe3O4 nanoparticles, and the amount of ATP was detected with luciferase chemiluminescence method. Meanwhile, the amount of ATP of lactic acid bacteria in commercially yogurt was tested, which had high sensitivity and good reproducibility. The results showed that these functionalized-Fe3O4 nanoparticles could act as good dispersion, easy separation carrier, which indicated uniform particle size, good stability, strong magnetism and excellent binding ability with ATP aptamer. The development of Fe3O4 nanoparticles largely expanded its application in the field of analysis.
Keywords: Fe3O4  nanoparticles  aptamer  ATP  chemiluminescence detection
投稿时间:2016-08-31 修订日期:2016-11-21
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