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| A Highly Sensitive Ratiometric Fluorescence Probe for Zn2+ Detection in Living Cells |
| Author Name | Affiliation | E-mail | | ZHANG Chang-Li | School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China | carbon314@163.com | | XU Jian | School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China | | | HUANG Fang | School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China | | | ZHAO Hai-Rong | School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China | | | CHEN Chang-Yun | School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China | |
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| Abstract: The emission spectra of 2-PBI (2-(2'-pyridyl) benzimidazole) show a red shift at a specific wavelength in both aqueous and acetonitrile solutions. However, 2-PBI cannot be used as ratiometric fluorescent probe for Zn2+ because of its low binding ability and variable coordination ratio to Zn2+. In this study, in order to add coordination atoms and promote the ICT effect of the probe by introducing N,N-dimethyl group at its 5-position of 2-PBI, design and synthesize a new ratiometric Zn2+ fluorescent probe DBITA. The results show that, besides large Stokes shift of 172 nm, DBITA exhibits the specific Zn2+-induced emission red-shift from 534 nm to 609 nm. DBITA combines with Zn2+ as 1:1 binding ratio. Moreover, DBITA displays the extremely high affinity toward Zn2+ of 0.16 pmol·L-1. In HeLa cells, DBITA can be used to quantitatively detect intracellular Zn2+. |
| Keywords: zinc ions fluorescence probe ratiometric quantitative cell imaging |
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| ZHANG Chang-Li,XU Jian,HUANG Fang,ZHAO Hai-Rong,CHEN Chang-Yun.A Highly Sensitive Ratiometric Fluorescence Probe for Zn2+ Detection in Living Cells[J].Chinese Journal of Inorganic Chemistry,2020,36(1):97-105. |
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