| 金属—血清白蛋白的结构研究——Ⅵ.等离子点附近HSA和BSA中Cu(Ⅱ)和Ni(Ⅱ)金属中心的结构 |
| STRUCTURAL STUDIES ON METAL-SERUM ALBUMIN VI. STRUCTURE OF THE Cn(Ⅱ) AND Ni(Ⅱ) METAL CENTER IN HSA AND BSA NEARBY THE ISOIONIC POINT |
|
| 摘要: 本文用紫外光谱研究了等离子点附近HSA或BSA 1Cu(Ⅱ)或Ni(Ⅱ)金属中心的结构。结果表明:在pH4.0～5.3时,Cu(Ⅱ)—HSA配合物在低浓度时独具五配位的四方锥构型,高浓度时(>10-4mol·l-1)为四配位的四方平面构型,Cu(Ⅱ)—BSA、Ni(Ⅱ)—BSA在上述pH范围内均只存在四方平面构型。Cu(Ⅱ)、Ni(Ⅱ)结合位置与生理pH下的相同,均在白蛋白的N端三肽段上,与Asp1 |
| 关键词: 铜(Ⅱ)—血清白蛋白 镍(Ⅱ)—血清白蛋白 构型 光学电负性 pH效应 |
| 基金项目: |
| Abstract: In this paper, the structure of the Cu(Ⅱ) or Ni(Ⅱ) metal center in HSA or BSA nearby the isoionic point have been studied by ultraviolet spectroscopy. The results show in pH 4.0-5.3 range, the configuration of Cu(Ⅱ)-HSA complexes is uniquely the pcntacoordinated square-pyramidal at concentrations lower than 4.0 × 10-4mol·l-1. But at concentrations greater than 4.0 × 10-4mol·l-1, it takes the tctracoordinated square-plannar form. In above pH range, the configuration of Cu(Ⅱ)-BSA, Ni(Ⅱ)-BSA are only the tetracoordinated square-plannar. The binding sites of Cu(Ⅱ), Ni(Ⅱ) are same as those at physiological pH, they are all located at the N-terminal sequence of HSA or BSA, and coordinated with -NH2, imidazoyl and two pcptide nitrogens. In Cu(Ⅱ)-HSA, the fifth binding group is COO- of Asp1. Above pH effect has also been discussed. |
| Keywords: Cu(Ⅱ)-scrum albumin Ni(Ⅱ)-serum albumin configuration optical electronegativity pH effect |
| 投稿时间：1991-03-13 |
| 摘要点击次数: 1478 |
| 全文下载次数: 1361 |
| 周永洽,梁宏,郝韵琴,申泮文.金属—血清白蛋白的结构研究——Ⅵ.等离子点附近HSA和BSA中Cu(Ⅱ)和Ni(Ⅱ)金属中心的结构[J].无机化学学报,1992,8(4):382-386. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| Support information: |
|
|
|
|
|
|
