| 外源Cu2+与烟草CuZnSODⅢ的相互作用 |
| The Interaction of CuZnSODⅢ and Outer-Copper |
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| 摘要: |
| 关键词: CuZnSODⅢ Cu2+ 相互作用 荧光光谱 烟草 |
| 基金项目: |
| Abstract: In this paper， we have studied the interaction of CuZnSODⅢ and Outer-Copper， enzyme activity experiments expressed that 0.1mmol·L-1 Cu2+ addition reduced the enzyme activity sharply， but this reduced action had not been found for the additions of 0.1mmol·L-1 1∶1 Cu2+ and Zn2+， and 0.1mmol·L-1 Zn2+， respectively. This was due to the Cu2+ exchanged the Zn2+ in CuZnSODⅢ，and it was proved by the experiment of determination of metal content. Meanwhile， the static fluorescence quenching mechanism revealed the exist of molecular complex of CuZnSOD with Cu2+. The binding constant was obtained from lineweaver-burk and double-lg plot. The distance of active site to Trp is about 2.83nm， was calculated according to F?rster theory. |
| Keywords: CuZnSODⅢ Cu2+ interaction fluorescence spectrum tobacco |
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| 盛良全,刘少民,叶纪华,徐小龙,解永树,刘清亮.外源Cu2+与烟草CuZnSODⅢ的相互作用[J].无机化学学报,2003,19(2):191-195. |
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