| 二维DNA晶体的多重熔解过程研究 | |
| The Melting behavior of 2D-DNA Lattices | |
| 摘要: 可人工编程设计的刚性DNA分子瓦(DNA tile)中的DX分子(double-crossover，双交叉)能自组装形成二维DNA晶体。将含有二维DNA晶体的缓冲溶液程序升温，用紫外-可见分光光度计在260 nm处测定二维DNA晶体的熔解曲线，观测到了DNA晶体的多重熔解过程。AFM显微镜的研究也观测到了二维DNA晶体受热解体后的图像，表明二维DNA晶体的熔解过程首先发生在DX分子瓦间的黏性末端，然后是DX分子瓦的解体，由此推测，在DNA晶体生长过程中单链DNA相互结合成分子瓦后，分子瓦进一步自组装成晶体。 | |
| 关键词: 二维DNA晶体 DNA分子瓦 紫外升温扫描 AFM | |
| 基金项目: | |
| Abstract: The synthetic sequences are designed to self-assemble into four double-crossover (DX) DNA tiles. The ‘sticky ends’ of these tiles that associate according to Watson-Crick′s base pairing are programmed to build up specific periodic patterns upto tens of microns. UV thermal scans were carried out with increasing the temperature of the 2D-DNA buffer solution. Melting curves were collected at 260 nm. According to the melting process and AFM imaging, we infer that the DNA lattices were first split into 4 DNA DX tiles, then into single oligonucleotides. It is proposed from the melting process that the 22 oligonucleotides self-assemble into four DNA tiles, then into the 2D-DNA crystal. | |
| Keywords: 2D-DNA crystal DNA tiles DNA melting curves AFM | |
| 投稿时间：2004-09-01 修订日期：2004-10-12 | |
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| 宋琤,陈亚清,刘小鹏,魏帅,王晶,肖守军,游效曾.二维DNA晶体的多重熔解过程研究[J].无机化学学报,2005,21(1):21-24. | |
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