葡萄糖氧化酶在活性炭上的固定及直接电化学
Direct Electrochemistry of Glucose Oxidase Immobilized on Surface of Activated Carbon
作者单位
孙冬梅 中国科学院长春应用化学研究所长春 130022;南京师范大学化学与环境科学学院南京 210097 
蔡称心 南京师范大学化学与环境科学学院南京 210097 
邢巍 中国科学院长春应用化学研究所长春 130022 
陆天虹 中国科学院长春应用化学研究所长春 130022;南京师范大学化学与环境科学学院南京 210097 
摘要: 
关键词: 直接电化学  生物电催化  葡萄糖氧化酶  活性炭
基金项目: 
Abstract: The glucose oxidase (GOD) immobilized onto the surface of activated carbon powders at the glassy carbon electrode (GOD-C/GC) could undergo the quasi-reversible, direct electrochemical reaction. Its formal redox potential, E0′, is almost independent on the scan rates. The average value of E0′ is (-0.467 ± 0.002) V (vs SCE) in the pH 6.8 phosphate buffer solution. Its apparent heterogeneous electron transfer rate constant (ks) is (1.18 ± 0.59) s-1, which is much higher than that reported previously. The dependence of E0′ on the pH of the buffer solution indicated that the direct electrochemical reaction of the immobilized GOD is a two-electron transfer reaction process coupled with two-proton transfer. The further experimental results demonstrated that the immobilized GOD retained its bioelectrocatalytic activity to the oxidation of β-D(+) glucose.
Keywords: direct electrochemistry, bioelectrocatalysis, glucose oxidase, activated carbon powder
投稿时间:2004-07-20 修订日期:2004-12-01
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孙冬梅,蔡称心,邢巍,陆天虹.葡萄糖氧化酶在活性炭上的固定及直接电化学[J].无机化学学报,2005,21(3):405-408.
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