| 硅钨杂多酸与牛血红蛋白相互作用的研究 |
| Interaction between Silicotungstic Heteropolyacid and Bovine Hemoglobin (BHb) |
|
| 摘要: |
| 关键词: 硅钨杂多酸 牛血红蛋白 紫外光谱 荧光光谱 热力学参数 |
| 基金项目: |
| Abstract: The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a major role in stabilizing the complex. The effect of silicotungstic heteropolyacid on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy. |
| Keywords: silicotungstic heteropolyacid BHb UV-Vis spectroscopy fluorescence spectroscopy thermodynamic parameters |
| |
| 摘要点击次数: 1161 |
| 全文下载次数: 2012 |
| 王彦卿,张红梅,张根成.硅钨杂多酸与牛血红蛋白相互作用的研究[J].无机化学学报,2006,22(5):895-899. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| Support information: |
|
|
|
|
|
|
