| 带羟乙基侧臂三脚架多胺Cu(Ⅱ)配合物切割肌红蛋白所得片断的质谱指认(英文) |
| Mass Spectrometry Assisted Assignments of Fragments of Myoglobin Cleaved by Copper(Ⅱ) Complex with Tripodal Polyamimine Bearing an Hydroxyethyl Pendant |
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| 摘要: 采用电喷雾质谱和串联质谱以及聚丙烯酰胺凝胶电泳技术研究了[CuL(H2O)](BF4)2(L为2-[二(2-氨乙酸)氨基]乙醇)与马心肌红蛋白的键合作用和水解切割。聚丙烯酰胺凝胶电泳研究显示在中性及60 ℃条件下，切割效率与[CuL(H2O)]2+的浓度和温育时间密切相关。电喷雾质谱和串联质谱分析显示，[CuL(H2O)]2+通过与肌红蛋白的氨基酸His36，His93，His116和Arg139侧链的结合，并在羟乙基侧臂的促进下，选择性地水解了肽键Phe33-Thr34，Gln91-Ser92，Ala94-Thr95，His116-Ser117和Asn140-Asp141。 |
| 关键词: 肌红蛋白 质谱 Cu(Ⅱ)配合物 切割 |
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| Abstract: The bonding interaction and hydrolytic cleavage of horse heart myoglobin with [CuL(H2O)](BF4)2, where L is 2-[bis(2-aminoethyl)amino]ethanol, were investigated by electrospray ionization mass spectrometry(ESI-MS), tandem mass spectrometry(MS/MS) and SDS-PAGE electrophoresis. The SDS-PAGE electrophoresis showed that the cleavage yield was dependent on the concentration of [CuL(H2O)]2+ and incubation time. The ESI-MS and MS/MS analysis revealed that with the assistance of the pendant hydroxyl group in [CuL(H2O)]2+, [CuL(H2O)]2+ may selectively hydrolysis the peptide bonds of Phe33-Thr34, Gln91-Ser92, Ala94-Thr95, His116-Ser117 and Asn140-Asp141 of myoglobin by the binding of [CuL(H2O)]2+ to the side chains of His36, His93, His116 and Arg139 of myoglobin. |
| Keywords: myoglobin mass spectrometry Cu(Ⅱ) complex cleavage |
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| 蒋疆,赵春梅,洪瑾,唐惠炜,朱龙根.带羟乙基侧臂三脚架多胺Cu(Ⅱ)配合物切割肌红蛋白所得片断的质谱指认(英文)[J].无机化学学报,2007,23(2):243-252. |
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