| 杂多酸盐K7[PTi2W10O40]·6H2O与牛血清白蛋白相互作用的研究 |
| Interaction between Heteropoly Salt K7[PTi2W10O40]·6H2O and Bovine Serum Albumin |
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| 摘要: |
| 关键词: 杂多酸盐 牛血清白蛋白 荧光光谱 紫外光谱 热力学参数 同步荧光光谱 |
| 基金项目: |
| Abstract: Under the imitated physiological condition of animal body, the interactions of heteropoly salt (PM-19) with bovine serum albumin (BSA) were investigated by fluorescence and absorption spectroscopy. It was shown that this compound had a quite strong ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation, we found that BSA had reacted with PM-19 and formed a certain new compound. The quenching belonged to static fluorescence quenching. According to Lineweaver-Burk equation, the forming constants of the compound (298 K: 2.68 × 105 L·mol-1; 304 K: 2.19 × 105 L·mol-1; 310 K: 1.82 × 105 L·mol-1) and the thermodynamic parameters (ΔH=-24.72 kJ·mol-1; ΔS=20.97 J·mol-1·K-1 / 20.92 J·mol-1·K-1/ 20.97 J·mol-1·K-1; ΔG=-30.97 kJ·mol-1/ -31.08 kJ·mol-1/-31.22kJ·mol-1) at the corresponding temperatures were obtained. The latter shows that binding power between them is mainly electrostatic interaction. Based on F?rster′s non-radiation energy transfer mechanism, the binding locality (r=4.14 nm) was calculated between donor and accepter. The effect of PM-19 on the conformation of BSA was also analyzed by synchronous fluorescence spectroscopy. |
| Keywords: heteropoly salt BSA fluorescence spectroscopy absorption spectroscopy thermodynamic parameter synchronous fluorescence spectroscopy |
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| 梁彦秋,邓斌,刘婷婷,孙鹏,臧树良.杂多酸盐K7[PTi2W10O40]·6H2O与牛血清白蛋白相互作用的研究[J].无机化学学报,2007,23(4):688-692. |
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