| 色氨酸-镝(Ⅲ)配合物与鲱鱼精DNA的作用方式 |
| Binding Mode between Trp-Dy(Ⅲ) Complex and Herring Sperm DNA |
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| 摘要: 采用UV光谱法、荧光光谱法，在pH=7.40的缓冲溶液中确定了镝(Ⅲ)与色氨酸的结合比nDy(Ⅲ)∶nTrp=1∶3，Dy(Ⅲ)(Trp)3配合物与鲱鱼精DNA的结合比nDy(Ⅲ)(Trp)3∶nDNA=2∶1。用双倒数法确定了结合常数K25℃=5.75×104 L·mol-1和K37℃=3.27×104 L·mol-1。化学热力学研究显示配合物Dy(Ⅲ)(Trp)3与hsDNA的结合为熵和焓共同驱动。结合Scatchard法和粘度法，确定了配合物Dy(Ⅲ)(Trp)3与hsDNA之间主要为静电作用和嵌插作用。 |
| 关键词: 色氨酸 镝(Ⅲ) 鲱鱼精DNA 作用方式 |
| 基金项目: |
| Abstract: By means of UV and fluorescence spectra, the binding ratios between Dy(Ⅲ)(Trp)3 and DNA. in physiological pH environment(pH7.40) were determined as nDy(Ⅲ)(Trp)3∶nDNA=2∶1 and nDy(Ⅲ)∶nTrp=1∶3. The binding constant of K25℃=5.75×107 L·mol-1 and K37℃=3.27×105 L·mol-1 were obtained by double reciprocal method. Thermodynamic function computation demonstrates that ΔrSΘm and ΔrHΘm are the primary driving power of the interaction between Dy(Ⅲ)(Trp)3 and DNA. By combination analysis of the Scatchard and viscometry methods, we suggested that the interaction mode between Dy(Ⅲ)(Trp)3 complex and herring sperm DNA be electrostatic and intercalation bindings. |
| Keywords: tryptophan Dy(Ⅲ) herring sperm DNA binding mode |
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| 摘要点击次数: 2139 |
| 全文下载次数: 1993 |
| 赵 娜,王兴明,王 单,徐 妍,潘海专,丁立生.色氨酸-镝(Ⅲ)配合物与鲱鱼精DNA的作用方式[J].无机化学学报,2009,25(12):2129-2136. |
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