| 三元TBZ/HPB-铜(Ⅱ)-L-蛋氨酸配合物的合成、表征、抑菌活性及与DNA的作用 |
| Syntheses, Characterization, Antibacterial Activities of Ternary Copper(Ⅱ) Complexes with 2-(4′-Thiazolyl)benzimidazole/2-(2-pyridyl)benzimidazole and L-Methionine and Their Interaction with DNA |
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| 摘要: 本文合成了2个新的三元铜(Ⅱ)配合物：[Cu(TBZ)(L-Met)(H2O)]ClO4·H2O (1)和[Cu(HPB)(L-Met)]ClO4 (2)[TBZ=2-(4′-噻唑基)苯并咪唑，HPb=2-(2-吡啶)苯并咪唑，L-Met=L-蛋氨酸]。通过元素分析、摩尔电导率、IR、UV-Vis及电喷雾质谱对这些配合物进行了表征。用二倍稀释法研究了配合物的抗菌活性，发现配合物对金黄色葡萄球菌(Staphylococcus aureus，G+)，枯草杆菌(Bacillus subtilis，G+)，沙门氏杆菌(Salmonella，G-)和大肠杆菌(Escherichia coil，G-)具有良好的抑制作用。采用电子吸收光谱、荧光光谱、粘度测定及琼脂凝胶电泳方法研究了配合物与DNA的相互作用，结果表明，配合物以插入方式与DNA作用，在维生素C存在下通过羟自由基·OH，单线态氧1O2或者1O2类似物如Cu-O2,切割pBR322 DNA双螺旋结构。 |
| 关键词: 铜(Ⅱ)配合物 2-(4′-噻唑基)苯并咪唑 2-(2-吡啶)苯并咪唑 DNA 抑菌活性 |
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| Abstract: Two new ternary copper(Ⅱ) complexes: [Cu(TBZ)(L-Met)(H2O)]ClO4·H2O (1) and [Cu(HPB)(L-Met)]ClO4 (2) [TBZ=2-(4′-thiazolyl)benzimidazole, HPb=2-(2-pyridyl)benzimidazole, L-Met=L-methionine], were synthesized and characterized by elemental analysis, molar conductivity, IR , UV-Vis spectroscopy and ES-MS. The complexes were assayed against gram-positive (Staphylococcus aureus, Bacillus subtilis) and gram-negative (Salmonella, Escherichia coil) bacteria by doubling dilutions method, and the interaction of the complexes to DNA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and agarose gel electrophoresis. The results indicated that the complexes could bind to DNA by intercalative mode, and cleave pBR322 DNA in the presence of vitamin C in the involvement of the hydroxyl radical，and may be a singlet oxygen or a singlet oxygen-like entity such as copper-peroxide Cu-O2, with the order of the binding ability and cleavage activity of the complexes to DNA: complex 2>1. |
| Keywords: ternary copper(Ⅱ) complex 2-(4′-thiazolyl)benzimidazole 2-(2-pyridyl)benzimidazole DNA antibacterial activity |
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| 卢艳梅,区志镔,刘海峰,乐学义.三元TBZ/HPB-铜(Ⅱ)-L-蛋氨酸配合物的合成、表征、抑菌活性及与DNA的作用[J].无机化学学报,2011,27(4):704-710. |
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