| 2，6-二[(2′-甲酚基-2″-羟乙基)氨甲基]-对-甲酚合四锌(Ⅱ)促进DNA水解的研究 |
| DNA Hydrolysis Mediated by a Tetranuclear Zinc(Ⅱ) Complex with 2, 6-Bis {[(2-hydroxybenzyl)(2-hydroxyethyl)amino]methyl}-4-methylphenol |
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| 摘要: 本文利用圆二色、荧光光谱和琼脂糖凝胶电泳等方法考察了一个四核锌荧光配合物[ZnⅡ4(L-3H)2](ClO4)2·3.5H2O(I，L=2,6-二[(2′-甲酚基-2″-羟乙基)氨甲基]-对-甲酚)与DNA的结合性质及其DNA水解酶活性。结果表明，配合物I能通过与DNA磷酸骨架的共价结合诱导其构象变化，而本身的荧光被淬灭。该四核锌配合物能在生理条件和不加任何辅助剂情况下水解切割pBR322质粒DNA，且在60 μmol·L-1时表现出最大的切割效果，使DNA的水解速率提高了6~7个数量级。 |
| 关键词: 四核锌荧光配合物 DNA 水解切割 |
| 基金项目: |
| Abstract: DNA binding properties and hydrolase activity of a fluorescent tetranuclear Zinc(Ⅱ) complex [ZnⅡ4(L-3H)2](ClO4)2·3.5H2O (I, L=2, 6-bis{[(2-hydroxybenzyl)(2-hydroxyethyl)amino]methyl}-4-methylphenol), were monitored by circular dichroism (CD), fluorescence spectroscopy and agarose gel electrophoresis. The results indicate that I binds to phosphate backbones of DNA covalently. The interaction induces the conformational variation of DNA double helix and the fluorescence quenching of I. The complex can mediate rapid hydrolytic cleavage of pBR322 plasmid DNA under physiological conditions (pH=7.4) and in the absence of any external agents. And I exhibits its optimal DNA hydrolytic activity at the concentration of 60 μmol·L-1 and the rate of DNA hydrolysis was a 6~7-fold acceleration. |
| Keywords: fluorescent tetranuclear Zinc(Ⅱ) complex DNA hydrolytic cleavage |
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| 陈战芬,王晓勇,郭子建.2，6-二[(2′-甲酚基-2″-羟乙基)氨甲基]-对-甲酚合四锌(Ⅱ)促进DNA水解的研究[J].无机化学学报,2011,27(10):1945-1951. |
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