| 组氨酸钴氧合配合物二聚体形态的电喷雾串联质谱验证研究 |
| Confirmation of Dinuclear Oxygenated Cobalt-Histidine Complex by Electrospray Tandem Mass Spectrometry |
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| 摘要: 电喷雾质谱(ESI-MS)是一种软电离质谱技术，已在配合物的结构和机理的研究中显示了重要的作用。本文根据组氨酸钴(CoL2)(L=组氨酸)对分子氧活性很高，极易生成双核氧合配合物(CoL2-O2-CoL2)的特点，采用ESI-MS方法研究了组氨酸钴氧合物(CoL2-O2-CoL2)和组氨酸配合物ML2(M=Cu、Zn)。结果发现，质谱图中在相应于双核氧合配合物的高质荷比端(m/z>ML2)，CoL2出现质谱峰，而组氨酸配合物ML2(M=Cu、Zn)在质谱图中高质荷比端没有出现质谱峰，只有配合物ML2的相关峰；这个结果证明了文献报道中的双核氧合配合物(CoL2-O2-CoL2)的存在，根据所得质谱结果，初步研究了组氨酸钴双核氧合配合物和ML2配合物的裂解规律。结果表明，可根据质谱数据中有无二聚体形态，做出Co配合物有无吸氧性能的初步判断，因此电喷雾质谱(ESI-MS)可做为研究Co配合物氧合反应和表征Co氧合配合物的有效分析手段。 |
| 关键词: 电喷雾串联质谱 氧载体 组氨酸 钴配合物 二聚体氧合配合物 |
| 基金项目: |
| Abstract: ESI-MS is a kind of soft ionization mass spectrometric technique; it has proved to be a very important technique in the investigations on structures and mechanisms of complexes. In this paper a comparison study was conducted on the oxygenated cobalt complex of histidine (L) and copper/zinc complex of histidine in oxygen atmosphere since according to literatures among histidine complexes only histidine cobalt complex has a very high affinity to dioxygen and easily form a dinuclear oxygenated complexes (CoL2-O2-CoL2). The ESI-MS results showed that in mass spectrum, the fragment ions corresponding to the dinuclear oxygenated species in higher m/z range (m/z>ML2) were observed for CoL2 while for CuL2 and ZnL2 only fragment ions corresponding to ML2 were observed. Theses results confirmed the dinuclear oxygenated complexes (CoL2-O2-CoL2) existed in the CoL2 complex reported in literature. The possible fragmentation mechanism of CoL2 was proposed. Results from this study proved that ESI-MS could be used as a method to estimate preliminarily if a Co(Ⅱ) complex have the ability of uptaking dioxygen or not, and it could be used as an effective technique to research the oxygenation and characterize oxygenated Co(Ⅱ) complexes. |
| Keywords: electrospray tandem mass spectrometry oxygen carrier histidine cobalt complex dinuclear oxygenated complexes |
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| 付 佩,符继红,李俊芳,岳 凡,张旭龙,王吉德.组氨酸钴氧合配合物二聚体形态的电喷雾串联质谱验证研究[J].无机化学学报,2012,28(7):1360-1364. |
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