| 紫外光照下高铁肌红蛋白的还原 |
| Photoreduction of Metmyoglobin by Ultraviolet Irradiation |
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| 摘要: 实验中我们发现紫外波段光源照射高铁肌红蛋白(metmyoglobin，metMb)时，能发生与添加化学还原剂还原metMb相似的过程，本文采用紫外可见吸收光谱(UV-Vis)，圆二色光谱(CD)研究了metMb在特定紫外光源下的还原过程。580 nm和544 nm处还原峰的面积变化显示，metMb在光照时能被还原至MbFe(Ⅱ)H2O状态，且不同的光源、特定紫外定波长、温度、pH以及不同气体存在等条件下metMb的还原程度不同。在温度为10 ℃，偏碱性条件时，定波长254 nm照射有利于metMb还原；气体存在时，由于气体小分子与血红素铁的配位能力不同，不同气体对光照metMb还原的催化作用程度也有差异，CO和O2的存在对此过程有催化促进作用，这一结论在医学和生理学上有重要的意义。 |
| 关键词: 高铁肌红蛋白 紫外光照射 光照还原 紫外可见吸收光谱 气体小分子 |
| 基金项目: |
| Abstract: According to the experiment, we have discovered that the photoreduction procedure of metMb is similar to the chemical reduction course. In this research, the photoreduction induced by direct irradiation in the ultraviolet region was investigated by means of UV-Vis absorption and circular dichroism (CD) spectra. By comparing the changes of new absorption peak area at 580 and 544 nm of the Q band, we observed the reduction extent under different light sources, specific UV fixed wavelengths, temperature, pH values and gas exposure; metMb is reduced to MbFe(Ⅱ)H2O state after the illumination; the alkaline conditions are favored for reduction at 10 ℃ in 254 nm wavelength UV; as the coordination abilities of heme iron with gas molecules differ, the catalytic process of reduction varies under UV irradiation in the presence of gas. CO or O2 can facilitate the photoreduction process. These conclusions have a promised significance in medicine and physiology. |
| Keywords: metmyoglobin UV irradiation photoreduction UV-Vis absorption spectra gas molecules |
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| 安良梅,曹洪玉,唐 乾,郑学仿.紫外光照下高铁肌红蛋白的还原[J].无机化学学报,2012,28(7):1461-1468. |
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