| “Turn-on”型磷光Hcy/Cys纳米探针的制备与性质研究 | |
| “Turn-on” Phosphorescent Nanoprobes for Sensing Homocysteine and Cysteine | |
| 摘要: 在本工作中，我们以制备纯水相检测的纳米探针为目标，以二氧化硅纳米粒子作为载体，以含特异性检测基团的长发光寿命的磷光铱配合物作为信号单元，通过共价键接枝方法得到几种纳米探针。采用SEM、TEM、SAXRD和氮气吸附等表征方法对纳米探针的表面形貌和内部结构进行表征，并用磷光发射光谱研究基于不同载体所得到的纳米探针的光物理性质以及对高半胱氨酸(Hcy)和半胱氨酸(Cys)的响应性，最后通过理论计算对其响应机理进行探讨。实验结果表明，以MCM-41作为载体的杂化纳米探针具有更强的发光强度和更好的响应性，并且在纯水体系中实现了对半胱氨酸和高半胱氨酸的高选择性检测。 | |
| 关键词: 磷光纳米探针 二氧化硅纳米粒子 磷光重金属配合物 生物传感 | |
| 基金项目: | |
| Abstract: In this work, we designed and fabricated a series of new nanoprobes by using the high specific surface area and good biocompatibility of mesoporous silica nanoparticles as a probe carrier and the long lifetime luminescence of phosphorescent complexes as signal unit. The structures and physical properties of prepared nanoprobes were characterized by SEM, TEM, XRD and nitrogen adsorption/desorption isotherms. Spectrophotometric determination was performed in phosphate buffer saline(PBS) buffer for Hcy/Cys sensing. To further understand the response mechanism, the effect of the aldehyde(CHO) group on the photophysical properties was calculated by using TDDFT calculation method. The result demonstrated that the as-prepared nanoprobe exhibited high selectivity for Hcy and Cys in pure PBS, which provides the advantage in developing excellent phosphorescent cellular probes for practical applications. | |
| Keywords: silica nanoparticles phosphorescence nanoprobes iridium complexes biosensor | |
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| 刘湘梅,刘淑娟,杨会然,于海霞,汪静霞,翟 钊,赵 强,黄 维.“Turn-on”型磷光Hcy/Cys纳米探针的制备与性质研究[J].无机化学学报,2012,28(11):2271-2279. | |
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