| 含2-氨基吡啶及去甲基斑蝥酸根的钴(Ⅱ)配合物的晶体结构、与DNA和BSA相互作用及体外抗增殖活性(英文) | |
| Crystal Structure, Interaction with DNA and BSA and Antiproliferative Activities of Cobalt(Ⅱ) Complex with Demethylcantharate and 2-Aminopyridine | |
| 摘要: 溶液法合成了二(去甲基斑蝥酸根)合钴(Ⅱ)酸二(2-氨基吡啶鎓)配合物(Hapy)2[Co(DCA)2]·6H2O(DCA=去甲基斑蝥酸根，C8H8O5；Hapy=2-氨基吡啶鎓，C5H7N2)。通过元素分析、摩尔电导、红外光谱、热重分析和X-射线单晶衍射对配合物进行了结构表征。该配合物为三斜晶系P1 空间群，中心离子配位数为6。利用荧光光谱法和粘度法对配合物与DNA之间的相互作用进行了研究。结果表明，配合物以部分插入模式与DNA键合，猝灭常数Ksq为0.18。同时，利用荧光光谱研究了配合物与牛血清白蛋白(BSA)的相互作用。结果显示，配合物能与BSA发生较强的键合作用，键合常数Ka=3.88×106 L·mol-1。体外抗增值活性结果显示，配合物对人肝癌细胞(SMMC-7721)和人胃癌细胞(MGC80-3)的抑制活性均强于去甲基斑蝥酸钠。 | |
| 关键词: 去甲基斑蝥酸根 2-氨基吡啶 钴(Ⅱ)配合物 与DNA和BSA相互作用 | |
| 基金项目: | |
| Abstract: A cobalt(Ⅱ) complex (Hapy)2[Co(DCA)2]·6H2O (DCA=demethylcantharate, 7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylate, C8H8O5; Hapy=2-aminopyridinum, C5H7N2) was synthesized and characterized by elemental analysis, molar conductance, infrared spectra, thermogravimetric analysis and X-ray diffraction. The complex crys-tallized in the triclinic crystal system, P1 space group, with a=0.662 23(7) nm, b=1.016 41(11) nm, c=1.194 60(12) nm, V=0.792 49(14) nm3, Z=1, Dc=1.520 g·cm-3, Mr=725.57, λ(Mo Kα)=0.071 073 nm, F(000)=381, R=0.028 3 and wR=0.082 1 (I>2σ(I)). The coordination number of metal ion was six and DCA ions were tridentate ligand. DNA binding property of the complex was investigated by fluorescence spectra and viscosity measurements. Results indicated the complex could bind to DNA via partial intercalation mode. The value of quenching constant Ksq was 0.18. The interaction of the complex with bovine serum albumin (BSA) was also studied by fluorescence spectra. The results suggested that the complex could quench the fluorescence of BSA with the binding constant Ka of 3.88×106 L·mol-1. The antiproliferative activity test revealed that complex showed stronger inhibition ratios against human hepatoma cells (SMMC-7721) lines and human gastric cancer cells (MGC80-3) lines than Na2(DCA) in vitro. CCDC: 823705. | |
| Keywords: demethylcantharate 2-aminopyridine cobalt(Ⅱ) complex interaction with DNA and BSA | |
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| 张 帆,林秋月,郑博雯,胡益丹,郑晓诗.含2-氨基吡啶及去甲基斑蝥酸根的钴(Ⅱ)配合物的晶体结构、与DNA和BSA相互作用及体外抗增殖活性(英文)[J].无机化学学报,2012,28(11):2451-2457. | |
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