| 磺酸咔咯及其镓(Ⅲ)配合物与DNA的相互作用和光核酸酶活性 | |
| DNA Binding and Photonuclease Activity of Sulfonated Corrole and Its Gallium(Ⅲ) Complex | |
| 摘要: 本文用紫外-可见光谱、荧光光谱、圆二色光谱和粘度法研究了2,17-二(磺酸钠基)-5,10,15-三(五氟苯基)咔咯(1)及其镓配合物(1-Ga)与小牛胸腺DNA(ct-DNA)的相互作用。结果表明1和1-Ga通过外部结合的方式与ct-DNA相互作用,且结合能力1-Ga比1大。琼脂糖凝胶电泳实验显示1和1-Ga均具较好的光核酸酶活性,1-Ga光断裂DNA效果比1好,其光断裂机理与羟基自由基的产生有关。 | |
| 关键词: 磺酸咔咯 镓 DNA 光核酸酶 | |
| 基金项目: 国家自然科学基金(No.20971046, 21171057);广东省自然科学基金(No.10351064101000000)和中山大学光电材料与技术国家重点实验室开放基金资助项目。 | |
| Abstract: The interactions between 2, 17-bis(sodiumsulphonato)-5, 10, 15-tris(pentafluorophenyl)-corrole (1) and its gallium complex (1-Ga) with calf thymus DNA (ct-DNA) had been studied by UV-Vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity experiments. Results revealed that 1 and 1-Ga bind to ct-DNA via an outside(groove) binding mode and the DNA binding ability follows an order of 1-Ga>1. Agarose gel electrophoresis experiments showed 1 and 1-Ga display good photonuclease activity with 1-Ga having a better DNA photocleavage performance and hydroxyl radical is the reactive species involved in the photocleavage process. | |
| Keywords: sulfonated corrole gallium DNA photonuclease | |
| 投稿时间：2013-01-12 修订日期：2013-04-26 | |
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| 黄俊腾,张阳,王湘利,计亮年,刘海洋.磺酸咔咯及其镓(Ⅲ)配合物与DNA的相互作用和光核酸酶活性[J].无机化学学报,2013,29(8):1649-1656. | |
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