| 大尺寸SiO2大孔材料固定化漆酶 |
| Immobilization of Laccase on Large-Sized SiO2 Macroporous Materials |
| 作者 | 单位 | E-mail | | 张群 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | | | 张育淇 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | | | 刘晓贞 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | | | 梁云霄 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | liangyunxiao@nbu.edu.cn | | 张瑞丰 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | | | 龙能兵 | State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China | |
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| 摘要: 以表面固定Cu2+的改性大尺寸SiO2大孔材料作为载体,考察了时间、pH和给酶量对漆酶固定化效果的影响,并对固定化漆酶的活性和稳定性进行了研究。结果表明:5h时吸附达到平衡,pH为4.5、漆酶与载体比例为5mg·g-1时固定化效果最好,酶活回收率可达到100.4%;固定化漆酶的最适pH和最适温度较游离漆酶的均有升高且范围变宽,固定化后,漆酶的pH稳定性和热稳定性都得到显著提高;固定化漆酶的Km值略高于游离漆酶的;固定化漆酶具有良好的操作稳定性,与底物反应反复操作10批次后剩余酶活为72.7%。 |
| 关键词: 漆酶 固定化 大尺寸 SiO2大孔材料 |
| 基金项目: 浙江省自然科学基金(No.LY12B01004),宁波市社会发展项目(No.2011C50052),宁波大学王宽诚幸福基金(No.XKL072),先进能源材料化学教育部重点实验室(南开大学)开放课题(No.KLAEMC-OP201201)资助项目。 |
| Abstract: Alarge-sized SiO2 macroporous material modified by fixing Cu2+ on its surface was employed as the support for the immobilization of laccase. The effects of immobilization time, pHvalue and the amount of enzyme provided on the immobilization were investigated, and the activity and stability of the immobilized enzyme were also studied. The results show that the adsorption equilibrium is reached after about 5 hours. The optimum conditions for immobilization of laccase were at the immobilization pHof 4.5 and the ratio of enzyme to support of 5 mg·g-1, and in this case, the activity recovery of the immobilized laccase is 100.4%. Comparing with the free laccase, both the optimum working pHand temperature of the immobilized laccase are higher and the ranges are wider. The experiments show that the immobilization improves the pHstability and the thermal stability of laccase significantly. The Km of the immobilized laccase is higher than the free laccase's. The immobilized laccase has good operational stability, the remained activity is 72.7% after 10 times of reaction with the substrate. |
| Keywords: laccase immobilization large-sized SiO2 macroporous material |
| 投稿时间：2013-04-22 修订日期：2013-05-29 |
| 摘要点击次数: 1971 |
| 全文下载次数: 2109 |
| 张群,张育淇,刘晓贞,梁云霄,张瑞丰,龙能兵.大尺寸SiO2大孔材料固定化漆酶[J].无机化学学报,2013,29(10):2065-2070. |
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