| Ag+与Hg2+对G-四链体DNA结构的破坏及其对构筑DNA逻辑门的应用 |
| Disruption of G-Quadruplex DNA by Ag+ and Hg2+ and Its Application to DNA Logic Gate |
| 作者 | 单位 | E-mail | | 朱莉娜 | Department of Chemistry, Tianjin University, Tianjin 300072, China | linazhu@tju.edu.cn | | 王海仙 | Department of Chemistry, Tianjin University, Tianjin 300072, China | | | 孙润丰 | School of Foreign Languages, Qinghai University for Nationalities, Xining 810007, China | | | 李平 | Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Nankai University, Tianjin 300071, China | | | 孔德明 | Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Nankai University, Tianjin 300071, China | | | 李孝增 | Department of Chemistry, Tianjin University, Tianjin 300072, China | |
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| 摘要: 本工作利用圆二色光谱研究了Ag+与Hg2+对4种代表性G-四链体DNA结构的破坏作用。结果表明Ag+可能通过与碱基G螯合从而破坏G-四链体结构;Hg2 能通过形成T-Hg2+-T碱基对,及其他方式破坏G-四链体结构。含巯基(-SH)的半胱氨酸与Ag+与Hg2+可以发生较强的配位作用,从而使被Ag+与Hg2+破坏后的G-四链体DNA结构得以回复。基于此,一个新颖的Ag+/Hg2+-半胱氨酸-DNA逻辑门得以构筑。 |
| 关键词: G-四链体 DNA逻辑门 银离子 汞离子 半胱氨酸 圆二色光谱 |
| 基金项目: 国家自然科学基金(No.20801040,21175072)资助项目 |
| Abstract: The G-quadruplex-disrupting abilities of Ag+ and Hg2+ ions were investigated by utilizing the metal ions-mediated circular dichroism (CD) spectrum changes of four representative G-quadruplexes. The results show that Ag+ ion can disrupt G-quadruplexes by chelating to Gbases and thus can be used as a general G-quadruplex-disrupting agent. The results also suggest that Hg2+ ion may disrupt G-quadruplexes by formation of T-Hg2+-Tbase pairs as well as other species. The G-quadruplexes disrupted by Ag+ and Hg2+ can be recovered by cysteine (Cys), a thiol-containing amino acid, due to the tight binding interactions between Cys and the metal ions. Thus, the system by using Ag+ (or Hg2+) and Cys as two inputs and CDsignal as output can behave as a DNA IMPLICATIONlogic gate. |
| Keywords: G-quadruplex DNA logic gate Ag+ Hg2+ cysteine circular dichroism |
| 投稿时间：2013-03-01 修订日期：2013-04-19 |
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| 朱莉娜,王海仙,孙润丰,李平,孔德明,李孝增.Ag+与Hg2+对G-四链体DNA结构的破坏及其对构筑DNA逻辑门的应用[J].无机化学学报,2013,29(10):2215-2224. |
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