| 铽(Ⅲ)与PvdA作用的光谱研究 |
| Spectroscopic Studies on the Binding of Terbium to PvdA |
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| 摘要: Pyoverdine A(PvdA)是荧光假单胞菌分泌的一种水溶性较高的黄绿色荧光铁载体。在50mmol·L-1Tris-HCl，pH8.0条件下，使用紫外-可见吸收差光谱、荧光光谱研究了铽(Ⅲ)与荧光铁载体PvdA的结合。结果表明铽(Ⅲ)可与PvdA结合形成1：1的配合物，条件结合常数为(4.44±0.82)×1014mol-1·L。在生理条件下，PvdA可竞争伴清蛋白N-，C-端结合的铽(Ⅲ)形成Tb-PvdA配合物；Tb-PvdA与荧光假单胞菌细胞表面受体FpvA结合形成Tb-PvdA-FpvA复合物。 |
| 关键词: Tb3+ PvdA|光谱|蛋白质 |
| 基金项目: 山西省留学归国基金（No.201013）资助项目。 |
| Abstract: PvdA is a siderophore, secreted by Pseudomonas aeruginosa in order to get access to iron. PvdA in 50 mmol·L-1 Tris-HCl, pH8.0 was titrated with acidic solutions of Tb3+ and the binding of Tb3+ to PvdA was followed from changes both in the difference UV-Vis spectra at 380 nm and in fluorescence at 457 nm. The 1:1 stable complex of Tb-PvdA can be confirmed from spectral titration curves. Using EDTAas a competitor the conditional binding constant of the complex was measured to be (4.44±0.82)×1014 mol-1·L. The formed Tb-PvdA from the reaction between PvdA and Tb3+ saturated ovotansferrin can be followed by measuring the fluorescence emission of Tb3+ at 548 nm, demonstrating that the binding of PvdA for Tb3+ is stronger than that of apoovotransferrin. Furthermore, Tb-PvdA interaction with the PvdA-Fe outer membrane transporter, FpvA, of Pseudomonas aeruginosa could be followed by fluorescence resonance energy transfer between the tryptophans of FpvA (donor) and PvdA (acceptor). |
| Keywords: Tb3+ PvdA spectra protein |
| 投稿时间：2013-09-10 修订日期：2013-10-24 |
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| 张文龙,许彩虹,杨斌盛*.铽(Ⅲ)与PvdA作用的光谱研究[J].无机化学学报,2014,30(1):106-112. |
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