| 一种基于DPA识别基团的可视化检测锌离子的荧光增强型探针 |
| A Fluorescence Enhancement Probe for Visual Detection of Zn2+ with Receptor Based on DPA |
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| 摘要: 以N，N-二(2-吡啶甲基)胺(DPA)为识别基团，设计合成了一种用于检测Zn2+的荧光增强型探针WN，在CH3CH2OH/Tris-HCl(1：9，V/V，pH=7.4)缓冲溶液中研究了它对Zn2+的识别特性。实验结果表明，WN对Zn2+有较高的选择性和灵敏度，它们之间的结合比为1：1，对Zn2+的检出限为1.14×10-8mol·L-1。WN能够快速地可视化检测Zn2+，在HeLa活细胞中对Zn2+的荧光显微成像表明WN可应用于生物体的检测。 |
| 关键词: DPA 锌离子 荧光探针 可视化检测 荧光成像 |
| 基金项目: 国家自然科学基金资助项目(No.51173060)。 |
| Abstract: Afluorescence enhancement probe WNfor detection of Zn2+ with receptor based on di-2-picolylamine (DPA) was synthesized and structurally characterized. Its recognition properties with Zn2+ had been investigated in CH3CH2OH/Tris-HCl buffer solution (1:9, V/V, pH=7.4). The results showed that WN exhibited good sensitivity and selectivity to Zn2+, and the stoichiometry of WN-Zn2+ complex was estimated to be 1:1, the detection limit of Zn2+ was estimated to be 1.14×10-8 mol·L-1. WN was used for fast visual detection of Zn2+ and its intracelluar Zn2+ imaging in living HeLa cells, indicated that it could be applied in biological detection. |
| Keywords: DPA zinc ion fluorescent probe visual detection fluorescent imaging |
| 投稿时间：2013-10-13 修订日期：2013-12-09 |
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| 王维娜,郑元梅,陈雪梅.一种基于DPA识别基团的可视化检测锌离子的荧光增强型探针[J].无机化学学报,2014,30(4):872-878. |
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