| 一种应用于活细胞中检测Hg(Ⅱ)的苯并噻唑类荧光探针 |
| A Benzothiazole-Derived Fluorescent Probe for Detecting Hg(Ⅱ) in Live Cells |
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| 摘要: 合成和表征了一种苯并噻唑类的荧光探针(YH1),并用光谱法研究了它对不同金属离子的响应.结果表明:YH1对Hg2+显示出好的选择性和灵敏度,与Hg2+作用后,在紫外光的激发下它的溶液颜色由蓝色变为无色.在1.4~8.8μmol·L-1浓度的范围内,YH1的荧光强度与Hg2+浓度有线性关系,其对Hg2+的检出限为0.56μmol·L-1.此外,YH1可跨过细胞膜,细胞毒性低,还可应用于HeLa活细胞中对Hg2+进行荧光成像. |
| 关键词: 晶体结构 汞离子 荧光探针 苯并噻唑 荧光成像 |
| 基金项目: 湖北省教育厅B类项目(No.B20114404),矿区环境污染控制与修复湖北省重点实验室开放基金项目(No.2012103)和湖北省教育厅青年人才资助项目(No.Q20132901) |
| Abstract: Abenzothiazole-derived fluorescent probe (YH1) has been synthesized and structurally characterized, and its response to different metal ions has been investigated by spectrometry. The results showed that YH1 exhibited good sensitivity and selectivity to Hg2+. Its interaction with Hg2+ caused the color of its solution changed from blue to colorless under UVirradiation. There was a good linear relationship between the fluorescence intensity of YH1 and the concentration of Hg2+ in the range of 1.4 μmol·L-1 to 8.8 μmol·L-1, and its detection limit for Hg2+ was 0.56 μmol·L-1. Moreover, the cell membrane penetration of YH1 had low cytotoxicity, and it can be applied to image intracellular Hg2+ in living HeLa cells. CCDC: 978108. |
| Keywords: crystal structure Hg2+ fluorescent probe benzothiazole fluorescent imaging |
| 投稿时间：2014-08-29 修订日期：2014-10-25 |
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| 焦元红,张前,姜玉凤,张万举.一种应用于活细胞中检测Hg(Ⅱ)的苯并噻唑类荧光探针[J].无机化学学报,2015,31(2):361-368. |
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