| 中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离 | |
| Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein | |
| 摘要: 利用水热法合成了中空巯基纳米二氧化硅微球(SiO2-SH), 然后在其表面修饰亚氨基二乙酸基团(-IDA), 形成了中空SiO2-SH/IDA双功能化纳米微球。利用该纳米微球表面的-SH和-IDA双功能团, 可以更多的吸附溶液中的Ni2+, 形成SiO2-SH/IDA-Ni2+复合微球从而可以更好的分离以六聚组氨酸为标签的(His-tagged)蛋白。结果显示制备的样品对分离His-tagged蛋白具有广谱性, 并且具有较好的再生能力。 | |
| 关键词: 中空微球 SiO2 蛋白分离 功能化 亲和 | |
| 基金项目: 河南省教育厅科学技术重点研究项目(No.14B150003), 国家自然科学基金(No.21271062)资助项目。 | |
| Abstract: Hollow silica nanospheres with thiol groups (SiO2-SH NSs) have been successfully prepared through a hydrothermal route. Then the SiO2-SH NSs was further modified by conjugating iminodiacetic acid (IDA) to bear dual chelating groups (-SH and -COOH). After chelating Ni2+, these hollow NSs with dual chelating groups were applied to purify histidine-tagged (His-tagged) proteins. Results show that these hollow NSs can be widely used to separate His-tagged proteins and have a good reused ability. | |
| Keywords: hollow microspheres silica separation of protein functionalization affinity | |
| 投稿时间：2015-01-24 修订日期：2015-04-25 | |
| 摘要点击次数: 1763 | |
| 全文下载次数: 1895 | |
| 田淑芳,邹雪艳,卢海涛,何建英,陈丹云,赵彦保,郭静玉.中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离[J].无机化学学报,2015,31(7):1329-1334. | |
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