| 两个组氨酸希夫碱镍(Ⅱ)配合物的合成、晶体结构和DNA相互作用及SOD活性 |
| Syntheses, Crystal Structures, DNA Interactions and SOD Activities of Two Nickel(Ⅱ) Complexes with L-Histidine Schiff Base |
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| 摘要: 合成了2个新的L-组氨酸水杨醛希夫碱混配体镍(Ⅱ)配合物,[Ni(Sal-L-His)(Phen)]·H2O (1)(Sal-L-His=L-组氨酸水杨醛希夫碱,Phen=菲咯啉)和[Ni(Sal-L-His)(Bipy)]2·2CH3OH·3H2O (2)(Bipy=2,2'-联吡啶)。X射线单晶衍射测定表明,1为单核配合物,属于单斜晶系,P21/c空间群,晶胞参数为:a=1.58631(13) nm,b=1.21511(11) nm,c=1.21130(9) nm,β=106.581(2)°,V=2.2377(3) nm3,Z=4。2为双核配合物,属于正交晶系,C2221空间群,晶胞参数为:a=1.41530(13) nm,b=1.71771(15) nm,c=2.25602(19) nm,V=5.4846(8) nm3,Z=4。紫外吸收光谱、荧光光谱、圆二色光谱和粘度测定等研究表明,2个配合物与小牛胸腺DNA(CT-DNA)均以插入方式相结合。运用NBT光照还原法测定了配合物的超氧化物歧化酶(SOD)活性,求得IC50(1)=51.5μmol·L-1,IC50(2)=58.1μmol·L-1。 |
| 关键词: 镍配合物 L-组氨酸 希夫碱 晶体结构 DNA SOD |
| 基金项目: 山东省自然科学基金(No.Y2004B02)和聊城大学大学生科技文化创新基金(No.SF2014042)资助项目 |
| Abstract: Two new nickel(Ⅱ) complexes,[Ni(Sal-L-His)(Phen)]·H2O (1) (Sal-L-His=the Schiff base derived from salicylaldehyde and L-Histidine, Phen=1,10-phenanthroline), and[Ni(Sal-L-His)(Bipy)]2·2CH3OH·3H2O (2) (Bipy=2,2'-Bipyridine), have been synthesized. Their crystal structures have been determined by single crystal X-ray diffraction analysis. It showed that 1 is a mononuclear complex and belongs to monoclinic crystal system, P21/c space group with the cell parameters:a=1.58631(13) nm, b=1.21511(11) nm, c=1.21130(9) nm, β=106.581(2)°, V=2.2377(3) nm3, Z=4; 2 is a binuclear complex and belongs to orthorhombic crystal system, C2221 space group with the cell parameters:a=1.41530(13) nm, b=1.71771(15) nm, c=2.25602(19) nm, V=5.4846(8) nm3, Z=4. The results of UV absorption, fluorescence, circular dichroism(CD) spectra and viscosity measurement indicated that the two complexes bind to CT-DNA in an same intercalative mode. SOD-like activities of the complexes were determined by the modified photoreduction of NBT. The value of IC50(1) is 51.5μmol·L-1 and IC50(2) is 58.1μmol·L-1. |
| Keywords: nickel(Ⅱ) complex L-histidine Schiff base crystal structure DNA SOD activity |
| 投稿时间：2015-12-12 修订日期：2016-03-24 |
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| 魏强,董建方,李文彬,赵培然,丁菲菲,李连之.两个组氨酸希夫碱镍(Ⅱ)配合物的合成、晶体结构和DNA相互作用及SOD活性[J].无机化学学报,2016,32(5):789-798. |
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