| 基于苯并噻唑Zn2+荧光探针及其细胞造影应用 |
| Benzothiazole-Based Zn2+-Specific Fluorescent Probe and Its Cell Imaging Application |
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| 摘要: 设计合成基于苯并噻唑Zn2+荧光增强型探针BHP，在HEPES缓冲液中测其对Zn2+识别性能。实验结果表明，BHP对Zn2+有较高的选择性，对其他金属离子如Cd2+，Fe2+，Ni2+，Pb2+，Hg2+，Al3+，Mn2+，Ag+，Cu2+，Co2+，Na+，K+，Mg2+和Ca2+无明显荧光增强响应。BHP与Zn2+按1：1计量比配位，在生理条件下荧光强度不受pH值影响。在HeLa细胞中对Zn2+的造影表明BHP可用于生物体Zn2+检测。 |
| 关键词: 锌离子 镉离子 荧光探针 造影 |
| 基金项目: 国家自然科学基金（No.21401106）、江苏省自然科学青年基金（No.BK20140090）和南京晓庄学院校级科研项目（No.2013NXY03，2015XSKY078）资助。 |
| Abstract: A fluorescent probe 2-((2-(benzo[d]thiazol-2-yl)hydrazono)methyl)-5-(diethylamino)phenol (BHP) was designed and synthesized as a Zn2+ selective fluorescent chemosensor. It demonstrates Zn2+-specific emission enhancement, while many other ions such as Cd2+, Fe2+, Ni2+, Pb2+, Hg2+, Al3+, Mn2+, Ag+, Cu2+, Co2+, Na+, K+, Mg2+ and Ca2+, have no response. Notably, this chemosensor can distinguish Zn2+ from Cd2+. Its binding stoichiometry with Zn2+ is 1:1 ratio. Its pH-independent Zn2+-induced emission enhancement in physiological condition and cell permeability make it an effective intracellular Zn2+ imaging agent. |
| Keywords: zinc ion cadmium ion fluorescent sensor imaging |
| 投稿时间：2016-04-11 修订日期：2016-10-01 |
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| 张长丽,田佳津,邵阳,徐鉴.基于苯并噻唑Zn2+荧光探针及其细胞造影应用[J].无机化学学报,2016,32(12):2069-2074. |
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