| 在云母晶面上应用“自下而上”和“自上而下”两种途径制造胶原蛋白纳米线阵列 | |
| Creating Collagen Nanowire Arrays by ‘Bottom-Up’ and ‘Top-Down’ Approaches on Mica Lattice Planes | |
| 摘要: 以云母晶体的（001）晶面为基质，以天然Ⅰ型鼠尾胶原蛋白单体溶液为原料，分别考察了蛋白单体受基质规导进行“自下而上”的自组装过程，以及原子力显微镜探针对吸附在基质表面的蛋白膜层进行“自上而下”加工过程：（1）两种制备途径均能加工出结构精确可控的胶原蛋白纳米线阵列；（2）“自下而上”制备途径利用了蛋白单体和基质晶体在界面上互相识别并规范的“反相生物矿化”原理；（3）“自上而下”的加工利用了原子力显微镜接触模式下探针的“分子扫帚”机理。 | |
| 关键词: 胶原蛋白 自上而下 自下而上 纳米线 介观尺度 | |
| 基金项目: 国家自然科学基金（No.51303082），中央高校基本科研业务费专项资金（No.30915011316）和江苏省“高层次创新创业人才引进计划”资助项目。 | |
| Abstract: By using the mica (001) lattice plane as the substrate and rat tail type Ⅰ collagen monomers as adsorbates, both ‘bottom-up’ self-assembly of protein monomers guided by the substrate and ‘top-down’ manipulation of protein membrane by AFM tip were investigated:(1) both preparation approaches can fabricate collagen nanowire arrays with precisely controllable structures; (2) the ‘bottom-up’ approach was due to the ‘reverse-phase biomineralization’ mechanism arising from the cooperative recognition and regulation between the protein and substrate at the interface; (3) the ‘top-down’ manipulation was based on the ‘molecular broom’ mechanism of the AFM tip in the Contact Mode. | |
| Keywords: collagen bottom-up top-down nanowire mesoscale | |
| 投稿时间：2016-11-25 修订日期：2017-03-04 | |
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| 李力,张磊,杨德良,孙铭,曾凡喜,顾文华.在云母晶面上应用“自下而上”和“自上而下”两种途径制造胶原蛋白纳米线阵列[J].无机化学学报,2017,33(5):745-752. | |
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