| 一种用于活细胞中检测Zn2+的萘酚席夫碱类荧光探针 | |
| Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn2+ in Living Cells | |
| 摘要: 设计合成了1种基于C=N异构化和螯合荧光增强机理（CHEF）的Zn2+荧光探针BMO和NBMO，其结构经1H NMR，13C NMR，1H-1H COSY，HSQC，IR和HRMS进行了表征。光谱分析实验结果显示，探针对Zn2+均具有较好的选择性和灵敏度，检出限分别为30和21 nmol·L-1。在0~20 μmol·L-1浓度的范围内，BMO和NBMO的荧光强度与Zn2+浓度可呈良好的线性关系。NBMO-Zn2+配合物单晶结构和Job曲线证实该探针与Zn2+以1：1配位。NBMO被成功应用于活细胞中Zn2+的检测。 | |
| 关键词: 荧光探针 C=N异构化 螯合荧光增强 锌离子 活细胞成像 | |
| 基金项目: 国家自然科学基金（No.21472148，21072158），陕西省留学人员科技活动择优资助项目（No.20151190）和西北大学优秀青年学术骨干支持计划资助 | |
| Abstract: Two fluorescent probes BMO and NBMO were developed for the highly selective detection of Zn2+ based on C=N isomerization and chelation-enhanced fluorescence (CHEF) mechanism. Structure identification of the probes was confirmed by 1H NMR, 13C NMR, 1H-1H COSY, HSQC, IR and HRMS spectroscopy. The results of spectral analysis show that BMO and NBMO are sensitive and highly selective to Zn2+. The detection limit of BMO and NBMO are found to be 30 and 21 nmol·L-1 respectively. There is a good linear relationship between the fluorescence intensity of probes (BMO and NBMO) and the concentration of Zn2+ in the range of 0 to 20 μmol·L-1. The X-ray crystal structure of the NBMO-Zn2+ complex exposes its coordination feature and the Job plots reveal a 1:1 probe-Zn2+ identification. The cell images show that NBMO can be used to detect intracellular Zn2+. | |
| Keywords: fluorescent probe C=N isomerization chelation-enhanced fluorescence zinc ion living cell imaging | |
| 投稿时间：2017-05-23 修订日期：2017-09-01 | |
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| 陈邦,王少静,宋战科,郭媛.一种用于活细胞中检测Zn2+的萘酚席夫碱类荧光探针[J].无机化学学报,2017,33(10):1722-1730. | |
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