His-CDs@NaTbF4: Synthesis and Application in Detection of Histidine and Cancer Cell Imaging
刘玲 江南大学化学与材料工程学院, 无锡 214122  
李瑞怡 江南大学化学与材料工程学院, 无锡 214122  
来婷 江南大学化学与材料工程学院, 无锡 214122  
李在均 江南大学化学与材料工程学院, 无锡 214122 zaijunli@jiangnan.edu.cn 
顾志国 江南大学化学与材料工程学院, 无锡 214122  
摘要: 以组氨酸功能化碳点为稳定剂水热合成His-CDs@NaTbF4。所制备的NaTbF4为六方相晶体,碳点以共价键方式包覆于NaTbF4表面,粒子尺寸仅为4~6 nm。小尺寸和丰富亲水基团使复合材料易分散于水,形成的分散液具有良好的稳定性。碳点紫外-可见吸收光谱与NaTbF4的荧光发射光谱有较大程度重叠,且距离极短,因此碳点和NaTbF4的复合将导致荧光共振能量转移。与单独碳点相比,His-CDs@NaTbF4的荧光强度增加超过7倍。His-CDs@NaTbF4可与Cu2+配位而产生明显的荧光猝灭。然而,猝灭的荧光可被组氨酸恢复。基于以上行为,建立了一种"开-关"型组氨酸荧光检测方法。当组氨酸浓度在1.0×10-6~2.0×10-4 mol·L-1之间,峰荧光强度随组氨酸浓度增加而线性增大。该方法检出限为3.8×10-7 mol·L-1,已成功应用于人体尿液中组氨酸检测和Hela细胞成像。
关键词: 碳纳米材料  NaTbF4  光学响应  荧光共振能量转移  铜离子
基金项目: 国家自然科学基金(No.21176115)资助项目。
Abstract: Histidine-functionalized carbon dots(His-CDs) were used as a stabilizer for hydrothermal synthesis of His-CDs@NaTbF4. The formed NaTbF4 is a hexagonal crystal, its surface is covalently coated by carbon dots, and the crystal size is only in the range of 4~6 nm. The small size and rich of hydrophilic groups make the composite easy to disperse in water. The resulted dispersion is of excellent stability. As the absorption spectra of His-CDs and the fluorescence emission spectrum of NaTbF4 exist a great overlap as well a short distance between His-CDs and NaTbF4, their combination brings a remarkable fluorescence resonance energy transfer. The increase in the fluorescence intensity of the His-CDs@NaTbF4 is more than 7 times that of sole carbon dots. The coordination of Cu2+ with carbon dots on NaTbF4 surface results in an obvious fluorescence quenching process. However, the quenched fluorescence can be well recovered by adding histidine. Based on the above behaviors, one "On-Off" type fluorescence scheme was established for the detection of histidine. When the histidine concentration is in the range of 1.0×10-6~2.0×10-4 mol·L-1, the peak fluorescence intensity linearly increases with the increase of histidine. The analytical method gives a detection limit of 3.8×10-7 mol·L-1, and it has been successfully applied in determination of histidine in human urine and Hela cell imaging.
Keywords: carbon nanomaterials  NaTbF4  optical response  fluorescence resonance energy transfer  Cu2+
投稿时间:2018-01-23 修订日期:2018-04-05
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