兼具抗癌和药物递送作用的硒掺杂羟基磷灰石微球
Enhanced Antitumor Effect and Drug Delivery from Se Doped Hydroxyapatite Microspheres
作者单位E-mail
王艳华 三峡大学医学院, 宜昌 443002 wangyanhua@ctgu.edu.cn 
郝颃 华中科技大学先进生物材料与组织工程研究中心, 武汉 430074  
巫剑雄 三峡大学医学院, 宜昌 443002  
姚媛 三峡大学医学院, 宜昌 443002  
覃娜 三峡大学医学院, 宜昌 443002  
何文聪 三峡大学第一临床医学院, 宜昌 443002  
摘要: 利用碳酸钙作为处理模板,通过共沉淀联合水热法,制备了硒元素掺杂羟基磷灰石微球(HASe),期望硒掺杂能提高HA对溶菌酶的加载,并增强HASe微球的杀菌活性。所合成的HASe微球经SEM、TEM、DLS、XRD、FTIR和TGA测试对其理化性能进行了表征。并且利用姜黄素作为模式药物,评估了它们的药物加载及控释效能。结果发现,所合成的HASe产物为直径约1.0 μm的球体,球壁粘附有许多羟基磷灰石纳米棒(长约150 nm﹑宽约20 nm)。该HASe微球对姜黄素具有高的药物加载和缓慢稳定的控释效应。其药物加载量为(88.72±0.01)mg·g-1,在0~159 h内仅有不到1.5 mg的姜黄素被释放,且无爆释现象。此外,还通过血液分析和细胞实验评估了HASe微球的毒性行为。与无硒HA微球相比,HASe微球的血液毒性低,对细胞损伤少,然而对骨肉瘤细胞生长却具有强的抑制作用。
关键词: 羟基磷灰石    中空微球  药物递送系统  姜黄素
基金项目: 国家自然科学基金(No.81602559)资助项目。
Abstract: Selenium doped hydroxyapatite microspheres (HASe) were prepared by using self-sacrificing CaCO3 as template via hydrothermal route, and their physicochemical properties were characterized by SEM, TEM, DLS, XRD, FTIR and TGA. The curcumin was employed as the model drug, and its delivery behavior from HASe microspheres was evaluated with the loading efficacy and controlled-release efficiency. As a result, it was observed that the synthetic HASe products are spherical with an average diameter of about 1.0 μm. The surface of the microspheres is constructed by subtle units of hydroxyapatite nanorods which are 150 nm in length and 20 nm in width. HASe microspheres exhibit a high drug loading efficacy and slowly sustainable release behavior for curcumin. The loading amount of HASe10 was (88.72±0.01) mg·g-1, and less than 1.5 mg of curcumin was released from it during 0~159 h. There is no burst release but slow sustainable release manner in it. Moreover, the toxicity of HASe microspheres was investigated by blood assay and cell experiment. Compared with selenium-free HA microspheres, HASe microspheres show lower blood toxicity to healthy man, and less damage to normal cells, but stronger inhibition on the growth of the osteosarcoma cells.
Keywords: hydroxyapatite  selenium  hollow microspheres  drug delivery system  curcumin
投稿时间:2018-02-11 修订日期:2018-06-07
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王艳华,郝颃,巫剑雄,姚媛,覃娜,何文聪.兼具抗癌和药物递送作用的硒掺杂羟基磷灰石微球[J].无机化学学报,2018,34(8):1517-1530.
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