不同尺寸六边形一水草酸钙晶体的合成、表征、性质及其细胞毒性
Syntheses, Characterization, Properties and Cytotoxicity of Hexagonal Calcium Oxalate Monohydrate Crystals with Various Sizes
作者单位E-mail
郭达 暨南大学化学系, 生物矿化与结石病防治研究所, 广州 510632  
徐猛 暨南大学化学系, 生物矿化与结石病防治研究所, 广州 510632  
孙新园 暨南大学化学系, 生物矿化与结石病防治研究所, 广州 510632  
欧阳健明 暨南大学化学系, 生物矿化与结石病防治研究所, 广州 510632 toyjm@jnu.edu.cn 
摘要: 通过改变反应温度、溶剂、添加剂和搅拌速度,合成了尺寸分别为(1.5±0.4)、(3.5±0.5)、(5.5±0.5)和(9.5±1)μm的4种一水草酸钙(COM)晶体,比较了它们的理化性质差异。加入添加剂Na3Cit,增加搅拌速度,降低反应温度或降低溶剂的介电常数,均有利于获得小尺寸的晶体。X射线衍射(XRD)和傅立叶变换红外光谱(FT-IR)检测表明,这些晶体均为单相的COM晶体;扫描电子显微镜(SEM)观察显示,随着晶体尺寸由1 μm增大到9 μm,晶体由表面粗糙的圆钝形演变为表面光滑的薄片状,其高电荷密度的(101)晶面增大,晶体表面电荷密度增加,ζ电位绝对值也增大,形成的悬浮液稳定性越高。这些COM晶体对人肾近曲小管上皮细胞(HK-2)的损伤能力与其尺寸成负相关。
关键词: 一水草酸钙  尺寸效应  晶面  细胞毒性
基金项目: 国家自然科学基金(No.81670644)资助项目。
Abstract: Four kinds of calcium oxalate monohydrate (COM) crystals with the size of (1.5±0.4), (3.5±0.5), (5.5±0.5) and (9.5±1) μm respectively were synthesized by changing reaction temperature, solvent, additive and stirring speed. Their physiochemical properties were comparatively investigated. It is beneficial to obtain crystal with small size by adding additive Na3Cit, increasing stirring speed, reducing reaction temperature or decreasing dielectric constant of the solvent. The results of X-ray diffraction revealed that all these crystals are single-phase COM crystals. With the increase of crystal size from 1 to 9 μm, COM crystals changed from rough blunt round to smooth thin sheet, the (101) crystal face of COM with high charge density increased, and both the charge density and the absolute value of ζ potential on crystal surface increased, leading an increase of the stability of crystal suspension. The cytotoxicity of these COM crystals to human kidney proximal tubular epithelial (HK-2) cells is negatively correlated with the crystal size.
Keywords: calcium oxalate monohydrate  size effect  crystal face  cytotoxicity
投稿时间:2018-05-12 修订日期:2018-06-12
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郭达,徐猛,孙新园,欧阳健明.不同尺寸六边形一水草酸钙晶体的合成、表征、性质及其细胞毒性[J].无机化学学报,2018,34(10):1883-1890.
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