细胞内不同形态Gd基MRI造影剂的T1/T2弛豫性能及造影效果
T1/T2 Relaxation and MRI Contrast Effect of Gd Agent in Different Intracellular Forms
作者单位E-mail
孙兴丽 中国科学技术大学苏州纳米技术与纳米仿生学院, 合肥 230026
中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123 
 
张艳辉 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123  
赵敏敏 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123  
谭波 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123  
张海禄 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123  
邓宗武 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123 zwdeng2007@sinano.ac.cn 
摘要: 将具有良好生物膜穿透性的异烟肼(INH)和Gd-DO3A偶联,合成了小分子MRI造影剂Gd-DO3A-INH;利用脉冲电转染技术标记间充质干细胞,有效提高了进入细胞的Gd-DO3A-INH浓度,并诱导部分游离态Gd-DO3A-INH在细胞质中自组装成纳米粒子。细胞样品的TEM观察到细胞内形成了Gd-DO3A-INH纳米粒子;细胞传代实验和体外MRI揭示了2种不同状态的Gd-DO3A-INH对细胞水质子弛豫速率的影响机制,以及细胞传代过程中细胞内2种不同状态Gd-DO3A-INH的浓度涨落引起的MRI造影效果的变化机制。
关键词: 磁共振成像(MRI)  Gd基造影剂  弛豫速率  脉冲电转染标记
基金项目: 国家自然科学基金(No.21673281、31371010),中国科学院青年创新促进会(No.2012242)和中国科学技术部重点研究项目(No.2017YFA0104300)资助
Abstract: Isoniazid (INH) was coupled with Gd-DO3A to yield Gd-DO3A-INH as a small molecule MRI contrast agent with good membrane penetration activity. Pulsed electroporation was used to introduce Gd-DO3A-INH into the cytosol of human mesenchymal stem cells in two different forms:free Gd-DO3A-INH and Gd-DO3A-INH nanoclusters. Cellular TEM demonstrated the formation of intracellular Gd-DO3A-INH nanoclusters. Cell proliferation and cellular MRI demonstrated that the two different forms of Gd-DO3A-INH exerted different effect on the longitudinal (T1) and transverse (T2) relaxations of cellular water protons. Free Gd-DO3A-INH is in favor of acceleration of T1 relaxation, and exhibits fast exocytosis. Gd-DO3A-INH nanocluster is in favor of acceleration of T2 relaxation, and exhibits a promoted cell retention time. As a result, cellular MRI exhibits a fast recovery of T1 relaxation and T1-weighted signal intensity/contrast, and a delayed recovery of T2 relaxation and T2-weighted signal intensity/contrast during cell proliferation.
Keywords: magnetic resonance imaging (MRI)  Gd contrast agent  magnetic relaxivity  pulsed electroporation
投稿时间:2018-09-20 修订日期:2019-03-08
摘要点击次数:  198
全文下载次数:  155
孙兴丽,张艳辉,赵敏敏,谭波,张海禄,邓宗武.细胞内不同形态Gd基MRI造影剂的T1/T2弛豫性能及造影效果[J].无机化学学报,2019,35(5):780-786.
查看全文  查看/发表评论  下载PDF阅读器
Support information: 相关附件:   180519_Gd_基造影剂的细胞结合状态决定其_T1或T2_弛豫性能及_MRI_造影效果_附件_.doc