| 不同分子量黄芪多糖对草酸钙晶体生长的影响及其细胞毒性 |
| Effects of Astragalus Polysaccharides with Different Molecular Weights on Calcium Oxalate Crystal Growth and Cytotoxicity |
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| 摘要: 比较研究了羧基（-COOH）含量为16.7%（w/w），但分子量分别为11.0、8.4、4.7和2.6 kDa的4种黄芪多糖（ASP0、ASP1、ASP2和ASP3）对草酸钙（CaOx）晶体形成的调控作用。采用X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）检测表明，4种ASPs均可抑制COM生长，诱导COD生成。电感耦合等离子体发射光谱（ICP）检测表明，4种ASPs增加了溶液中可溶性Ca2+离子的浓度，同时减少了CaOx沉淀的生成量。ASPs还可以增加晶体表面的ζ电位绝对值，从而降低晶体的聚集程度。ASPs抑制CaOx生长和诱导COD的能力与其浓度呈正相关，与其分子量大小呈负相关，即分子量最小的ASP3调控能力最强。此外，各ASPs不仅对HK-2细胞没有毒性，还可以修复被草酸氧化损伤的HK-2细胞，降低细胞的ROS水平；其中分子量适中的ASP2修复能力最强。 |
| 关键词: 晶体生长 黄芪多糖 细胞毒性 分子量 结晶抑制剂 生物活性 |
| 基金项目: 国家自然科学基金（No.21975105）资助项目。 |
| Abstract: The effects of four astragalus polysaccharides (ASP0, ASP1, ASP2 and ASP3) with carboxylic group (-COOH) content of 16.7%(w/w) but molecular weights of 11.0, 8.4, 4.7 and 2.6 kDa, respectively, on the formation of calcium oxalate (CaOx) crystals were comparatively studied. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) results revealed that all the four ASPs inhibited COM growth, induced COD formation. The inductively coupled plasma emission spectra (ICP) revealed that these ASPs increased the concentration of soluble Ca2+ ions in the solution and reduced the amount of CaOx precipitation. Furthermore, ASPs increased the absolute value of ζ potential on the crystal surface, and thus inhibited crystal aggregation. The ability of ASPs to regulate CaOx crystal formation and induce COD formation is negatively related to its molecular weight, that is, ASP3 with the smallest molecular weight has the strongest regulatory ability. In addition, each ASPs is not only toxic to HK-2 cells, but also can repair oxalate-damaged HK-2 cells and decrease cellular ROS level, of which APS2 with moderate molecular weight showed the best repair ability. |
| Keywords: crystal growth astragalus polysaccharides cytotoxicity molecular weights crystallization inhibitor biological activity |
| 投稿时间：2019-11-20 修订日期：2020-04-22 |
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| 陈学武,黄芳,欧阳健明.不同分子量黄芪多糖对草酸钙晶体生长的影响及其细胞毒性[J].无机化学学报,2020,36(8):1437-1445. |
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