磁性凹凸棒土温敏性微凝胶的合成及其体外实验
Preparation and in Vitro Experiment of Attapulgite-based Microgels with Magnetic/Temperature Dual Sensitivities
作者单位E-mail
李天乐 南京工业大学化学与分子工程学院, 南京 210000
淮阴师范学院江苏低维材料化学重点实验室, 淮安 223300 
 
仲慧 南京工业大学化学与分子工程学院, 南京 210000
淮阴师范学院江苏低维材料化学重点实验室, 淮安 223300 
huizhong@hytc.edu.cn 
李小荣 淮阴师范学院江苏低维材料化学重点实验室, 淮安 223300 lxr206206@163.com 
周静 新疆农业大学食品科学与药学学院, 乌鲁木齐 830001
淮阴师范学院生命科学学院, 淮安 223300 
 
刘易鑫 淮阴师范学院江苏低维材料化学重点实验室, 淮安 223300  
胡卫成 淮阴师范学院生命科学学院, 淮安 223300  
程志鹏 淮阴师范学院江苏低维材料化学重点实验室, 淮安 223300  
姚成 南京工业大学化学与分子工程学院, 南京 210000 yaocheng@njtech.edu.cn 
摘要: 通过乳液聚合法制备了叶酸(FA)接枝的磁性FA-Fe3O4/凹凸棒土-聚(N-异丙基丙烯酰胺-丙烯酰胺)(FA-Fe3O4/ATP-P(NIPAM-AAM))复合微凝胶(凹凸棒土=ATP,N-异丙基丙烯酰胺=NIPAM,丙烯酰胺=AAM),并通过X射线衍射(XRD)、振动样品磁强计(VSM)、热重(TG)、红外分析(IR)、紫外可见分光光度仪(UV)、扫描电子显微镜(SEM)和透射电子显微镜(TEM)对其进行表征。通过动态光散射(DLS)测定的低临界溶液温度(LCST)约为38.5℃,该温度适合于细胞实验。选择盐酸阿霉素(DOX)作为模型药物。药物负载和释放试验表明,ATP可以增加药物的负载和释放量。体外细胞毒性实验表明,与游离DOX相比,负载DOX的FA-Fe3O4/ATP-P(NIPAM-AAM)具有更好的生物相容性,并有望建立一个药物缓释系统。体外细胞摄取实验表明,FA-Fe3O4/ATP-P(NIPAM-AAM)具有靶向性,可用于靶向药物释放。
关键词: 材料化学  磁性纳米复合材料  乳液聚合法  凹凸棒土  温度响应  靶向性  药物输送
基金项目: 国家自然科学基金(No.21775051,51676082,21405055)资助。
Abstract: The folic acid (FA)-grafted magnetic FA-Fe3O4/ATP-P(NIPAM-AAM) composite microgels (attapulgite=ATP, N-isopropyl acrylamide=NIPAM, acrylamide=AAM) were prepared via a method of emulsion copolymerization. The as-prepared microgels were characterized by X-ray diffraction (XRD), vibrating sample magnetometer (VSM), thermogravimetric analysis (TG), infrared spectroscopy (IR), UV-visible spectroscopy (UV), scanning electron microscope (SEM) and transmission electron microscope (TEM). The dynamic light scattering (DLS) results show that the low critical solution temperature (LCST) of microgel was about 38.5℃. The drug delivery ability of the as-prepared microgels was evaluated by using doxorubicin hydrochloride (DOX) as the model drug. Based on the drug loading and releasing results, the presence of ATP increased the amount of drug loading and releasing. Compared with free DOX, the in vitro cytotoxicity of DOX loaded FA-Fe3O4/ATP-P(NIPAM-AAM) was decreased and the biocompatibility was improved. Those results indicate that the microgels of FA-Fe3O4/ATP-P(NIPAM-AAM) can be used in potential as a slow-release drug system. The in vitro cellular uptake test revealed that the microgels of FA-Fe3O4/ATP-P(NIPAM-AAM) at the assigned site were significantly richer than that of other sites. This result indicates that the microgels of FA-Fe3O4/ATP-P(NIPAM-AAM) composite are targeting and expectable in the application of targeted drug releasing.
Keywords: materials chemistry  magnetic nanocomposites  emulsion polymerization  attapulgite  temperature-sensitive  targeted  drug delivery
投稿时间:2020-07-08 修订日期:2020-11-12
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李天乐,仲慧,李小荣,周静,刘易鑫,胡卫成,程志鹏,姚成.磁性凹凸棒土温敏性微凝胶的合成及其体外实验[J].无机化学学报,2021,37(1):180-188.
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