| 基于香豆素-肟的次氯酸根探针的设计、合成及荧光成像应用 |
| Design, Synthesis and Fluorescence Imaging Application of Hypochlorite Probe Based on Coumarin-oxime |
|
| 摘要: 通过将香豆素的2-位内酯转化为肟基，设计、合成了一种用于基于香豆素-肟类的次氯酸根荧光探针Cou-HC。Cou-HC不仅对次氯酸根表现出快速和高选择性响应的特点，而且探针氧化后的产物不会与生物硫醇等发生反应，从而可以避免生物硫醇对于次氯酸根响应的干扰。细胞成像实验结果表明，探针可以对RAW 264.7细胞中内源性和外源性次氯酸根进行实时成像，初步证明了该探针对生命体系内次氯酸根检测的能力。 |
| 关键词: 荧光探针 次氯酸根 香豆素 荧光检测 细胞成像 |
| 基金项目: 国家自然科学基金（No.21971115）资助。 |
| Abstract: We designed and synthesized a fluorescent probe for hypochlorite based on coumarin-oxime (Cou-HC). Not only Cou-HC showed a rapid and highly selective response to hypochlorite, but also the product after the probe oxidation did not react with biological thiols, which could avoid the interference of biological thiols on the response of hypochlorite. The results of cell imaging experiments showed that the probe could perform real-time imaging of endogenous and exogenous hypochlorite in RAW 264.7 cells, which proves the probe's ability to detect hypochlorite in living systems. |
| Keywords: fluorescent probe hypochlorite coumarin fluorescence detection cell imaging |
| 投稿时间：2021-01-22 修订日期：2021-04-27 |
| 摘要点击次数: 1182 |
| 全文下载次数: 607 |
| 张晶晶,严鸣,卢雯,徐莉,王小青.基于香豆素-肟的次氯酸根探针的设计、合成及荧光成像应用[J].无机化学学报,2021,37(6):1071-1079. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| Support information: 相关附件： file210035_ZJJ-SI-210323.doc |
|
|
|
|
|
|
