| 基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用 |
| Application of Fluorescent Probe Based on Dicyanoisophorone in Detection of Thiophenol |
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| 摘要: 为了开发具有高选择性和高灵敏度检测苯硫酚的方法，我们基于二氰基异佛尔酮染料优异的光学性能及良好的膜渗透性，以二氰基异佛尔酮衍生物作为荧光团，引入强吸电子基2，4-二硝基苯合成荧光探针（YC1）用于特异性识别苯硫酚。该探针对苯硫酚显示出明显的红色荧光（发射波长为594 nm，激发波长为420 nm），具有荧光增强（turn-on）的性质。探针对苯硫酚的识别具有良好的选择性且在PBS/DMSO（PBS=磷酸缓冲盐溶液，DMSO=二甲亚砜）的体系中检测限为0.65 μmol·L-1。此外，该探针已成功应用于HeLa细胞内4-甲基苯硫酚的成像。 |
| 关键词: 异佛尔酮 苯硫酚 荧光增强 生物成像 |
| 基金项目: 山西省高等学校教学改革创新项目（No.J2018192）和国家自然科学基金（No.21775096，22074084）资助。 |
| Abstract: To develop a method with high selectivity and sensitivity to detect thiophenol, based on the excellent optical properties and good membrane permeability of dicyanoisophorone dyes, we chose dicyanoisophorone derivatives as fluorophores and introduced a strong electron-withdrawing 2,4-dinitrobenzene synthetic fluorescent probe (YC1) for the specific recognition of thiophenol. The probe showed obvious red fluorescence on thiophenol (emission wavelength:594 nm, excitation wavelength:420 nm) and had a fluorescence-enhanced (turn-on) property for the recognition of thiophenol. The probe had a good selectivity for the recognition of thiophenol and the detection limit of the probe was 0.65 μmol·L-1 in PBS/DMSO system (PBS=phosphate buffer saline, DMSO=dimethyl sulfoxide). In addition, the probe has been successfully applied to the fluorescence imaging of 4-methylthiophenol in HeLa cells. |
| Keywords: dicyanoisophorone thiophenol fuorescence enhancement biological imaging |
| 投稿时间：2021-02-18 修订日期：2021-05-14 |
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| 张改清,阴彩霞.基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用[J].无机化学学报,2021,37(7):1245-1250. |
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| Support information: 相关附件： file210078_210078Supporting_Information.doc |
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