基于Pt/Au双金属修饰针灸针的非酶葡萄糖传感器
Non-enzyme Glucose Biosensor Based on Bimetallic Pt-Au Nanoparticles Decorated Acupuncture Needle
作者单位E-mail
肖琪 杭州电子科技大学自动化学院, 杭州 310018  
刘光献 杭州电子科技大学自动化学院, 杭州 310018  
陈简单 杭州电子科技大学自动化学院, 杭州 310018  
尹争志 嘉兴学院生物与化学工程学院, 嘉兴 314001 yinzhengzhi@mail.zjxu.edu.cn 
顾春川 杭州市肿瘤医院, 杭州 310002  
刘红英 杭州电子科技大学自动化学院, 杭州 310018 liuhongying@hdu.edu.cn 
摘要: 通过在不锈钢针灸针(AN)表面依次电沉积金(Au)纳米颗粒和铂(Pt)纳米颗粒,基于它们在AN表面的协同作用,实现了一种用于非酶葡萄糖检测的电化学生物传感器。首先,通过扫描电子显微镜对其功能界面(Pt/Au/AN)进行表征,结果显示类似卷心菜的纳米材料均匀致密地分布在AN表面。然后,通过循环伏安法和电化学阻抗法对Pt/Au/AN电极的电化学特性进行了研究。结果表明,与Au/AN或Pt/AN电极相比,Pt/Au/AN电极对葡萄糖氧化表现出优越的电催化活性。这表明双金属Pt/Au的接触界面是葡萄糖氧化的重要电催化位点。在pH7.4的模拟生理介质中,制得传感器的线性范围为0.1~35 mmol·L-1,检测限为0.0763 mmol·L-1,对葡萄糖的检测表现出较高的灵敏度和良好的抗干扰性能、稳定性。此外,该传感器已成功用于人体血清葡萄糖的检测。
关键词: 针灸针  Pt/Au双金属接触界面  非酶传感器  葡萄糖
基金项目: 浙江省科技计划项目(No.LGF22H200012,LGC20H200001,LGF22B050008)、国家自然科学基金资助项目(No.2020KY727)和浙江省医药卫生技术发展计划项目(No.2017AY33086)资助
Abstract: An electrochemical biosensor for non-enzyme glucose detection was constructed based on the synergistic action of gold (Au) nanoparticles and platinum (Pt) nanoparticles on the surface of a stainless steel acupuncture needle (AN), which was achieved by respectively electrodepositing. The functional interface (Pt/Au/AN) was characterized by a scanning electron microscope, showing that cabbage-like nanomaterials were uniformly and densely distributed on the surface of AN. Pt/Au/AN electrode also possessed outstanding electrochemical characteristics, which were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Significantly, Pt/Au/AN electrode exhibited remarkably electrocatalytic activity toward glucose oxidation compared with Au/AN or Pt/AN electrode. The results indicated that the contact interface of bimetallic Pt/Au was the vital electrocatalytic site for glucose oxidation. A further study proved that the contact interface exhibited intrinsic features and distinct selectivity for sensing glucose. The prepared sensor showed a wide linear range from 0.1 to 35 mmol·L-1, and the detection limit of glucose was 0.076 3 mmol·L-1. The sensor showed great stability, excellent selectivity, and miniaturization. Furthermore, the sensor was successfully used for the detection of glucose in human serum.
Keywords: acupuncture needle  contact interface of bimetallic Pt/Au  non-enzyme sensor  glucose
投稿时间:2021-12-18 修订日期:2022-04-22
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肖琪,刘光献,陈简单,尹争志,顾春川,刘红英.基于Pt/Au双金属修饰针灸针的非酶葡萄糖传感器[J].无机化学学报,2022,38(6):1159-1170.
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