过饱和度、钙/草酸化学计量比对草酸钙晶体形成的影响及降解茯苓多糖的调控作用
Effects of supersaturation, Ca2+/C2O42- stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide
作者单位E-mail
郑雨云 暨南大学化学与材料学院, 生物矿化与结石病防治研究所, 广州 510632  
刘静鸿 暨南大学化学与材料学院, 生物矿化与结石病防治研究所, 广州 510632  
陈佳云 暨南大学化学与材料学院, 生物矿化与结石病防治研究所, 广州 510632  
欧阳健明 暨南大学化学与材料学院, 生物矿化与结石病防治研究所, 广州 510632 toyjm@jnu.edu.cn 
摘要: 为研究草酸钙(CaC2O4)晶体成核、生长和聚集,探讨降解茯苓多糖(PCP)的抑制作用,采用X射线衍射、FT-IR、扫描电镜、拉曼光谱、ζ电位仪和紫外分光光度计等方法对不同条件下形成的CaC2O4晶体进行表征。结果表明,在低过饱和度(RS ≤ 26.6)时,主要生成一水草酸钙(COM)晶体;至RS为37.6和46.0时分别生成了11.6%和38.3%的二水草酸钙(COD)晶体,且高RS时晶体的聚集程度增加。在RS相同时,随着Ca2+/Ox2-(Ox2-=C2O42-)化学计量比(nCa2+/nCa2-)增大,晶体中COD比例增加。降解PCP的加入可增加体系中可溶性Ca2+浓度,减少生成的CaC2O4晶体质量,增加晶体表面ζ电位绝对值,这些均有利于抑制CaC2O4结石的形成。因此,高Ox2-浓度对肾结石形成的风险远大于高Ca2+浓度,提示草酸的摄入对CaC2O4结石的风险远远大于钙的摄入,降解PCP能同时抑制CaC2O4晶体的成核、生长和聚集。
关键词: 草酸钙  茯苓多糖  Ca2+/Ox2-化学计量比  过饱和度  晶体调控
基金项目: 国家自然科学基金(No.21975105)资助。
Abstract: To study the nucleation, growth, and aggregation of calcium oxalate (CaC2O4) crystals, and to explore the inhibitory effect of degraded Poria cocos polysaccharide (PCP), CaC2O4 crystals formed under different conditions were characterized by X-ray diffraction, FT-IR, scanning electron microscope, Raman spectroscopy, ζ potentiometer, and UV spectrophotometer. The results showed that calcium oxalate monohydrate (COM) crystals were mainly formed at low supersaturation (RS ≤ 26.6). At RS being 37.6 and 46.0, 11.6% and 38.3% calcium oxalate dihydrate (COD) crystals were formed respectively, and the aggregation degree of the crystals increased at high RS. At the same RS, the proportion of COD in the crystal increased with the increase of the Ca2+/Ox2- stoichiometric ratio (nCa2+/nCa2-, Ox2-=C2O42-). The addition of degraded PCP could increase the concentration of soluble Ca2+ ions in the system, reduce the quality of generated CaC2O4 crystals, and increase the absolute value of ζ potential on the crystal surface, all of which are beneficial to inhibit the formation of CaC2O4 stones. Thus, the risk of high Ox2- concentration on the formation of kidney stones is much greater than that of high Ca2+ concentration, suggesting that the risk of oxalic acid intake on CaC2O4 stones is much greater than that of calcium intake. PCP can simultaneously inhibit the nucleation, growth, and aggregation of CaC2O4 crystals.
Keywords: calcium oxalate  Poria cocos polysaccharide  Ca2+/Ox2- stoichiometric ratio  supersaturation  crystal regulation
投稿时间:2022-07-19 修订日期:2023-03-15
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郑雨云,刘静鸿,陈佳云,欧阳健明.过饱和度、钙/草酸化学计量比对草酸钙晶体形成的影响及降解茯苓多糖的调控作用[J].无机化学学报,2023,39(5):794-804.
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