| 吡咯腙对溶酶体中Hg2+的荧光成像 | |
| Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes | |
| 摘要: 合成了一种新的吡咯腙探针1,用于Hg2+的比色和荧光开启检测。探针1对Hg2+的检测限为45 nmol·L-1,缔合常数为5.78×108 L·mol-1。值得注意的是,工作pH范围为4.0~10.0。Job曲线和MS数据证实探针与Hg2+形成1:1的配合物。通过1H NMR、时间分辨荧光光谱和密度泛函理论(DFT)计算系统研究了探针与Hg2+的配位模式。此外,由于吗啉基团的存在,探针可以检测HeLa细胞溶酶体中的Hg2+。 | |
| 关键词: 细胞成像 荧光探针 Hg2+ 腙 溶酶体 吡咯 | |
| 基金项目: 国家自然科学基金(No.21907023，21001040，61964008)、河南省重点研发与推广专项科技攻关项目(No.232102320092)、江西省自然科学基金(No.20212BAB204017)、河南省高校基本科研业务费项目(No.NSFRF230402)、河南理工大学“双一流”创建工程项目(No.AQ20230745，AQ20230754)和河南理工大学研究生教改项目(No.2021YJ16)资助。 | |
| Abstract: A novel pyrrole-based hydrazone has been synthesized for colorimetric and fluorescent turn-on detection of Hg2+ ions. A 1:1 binding ratio of the Hg2+ complex has been obtained from Job's plots and MS data. The coordination mode was systematically investigated by 1H NMR, time-resolved fluorescence spectroscopy, and density functional theory (DFT) calculations. The limit of detection of 1 for Hg2+ was as low as 45 nmol·L-1 with an association constant of 5.78×108 L·mol-1. It is worth noting that the response of 1 to Hg2+ was good in a pH range of 4.0 to 10.0. In addition, owing to the existence of the morpholine group, the probe can detect lysosomal Hg2+ in HeLa cells. | |
| Keywords: cell imaging fluorescent probe Hg2+ hydrazone lysosome pyrrole | |
| 投稿时间：2022-04-03 修订日期：2023-04-27 | |
| 摘要点击次数: 2682 | |
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| 王震,李思媛,王元,吴伟娜,张磊,陈忠,闫玲玲.吡咯腙对溶酶体中Hg2+的荧光成像[J].无机化学学报,2023,39(6):1122-1130. | |
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| Support information: 相关附件： 220160_supporting_information.doc | |
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