| 叶酸稳定的银纳米团簇对孔雀石绿的有效检测 | |
| Effective detection of malachite green by folic acid stabilized silver nanoclusters | |
| 摘要: 以叶酸(FA)为保护剂、水合肼为还原剂、硝酸银为前驱体，通过一锅法制备了FA稳定的银纳米团簇(FA@Ag NCs)。分别采用透射电子显微镜(TEM)、X射线光电子能谱仪(XPS)、傅里叶变换红外光谱仪(FTIR)、X射线衍射仪(XRD)、荧光光谱仪和紫外可见吸收光谱仪对FA@Ag NCs的结构和光学性质进行分析。FA@Ag NCs呈现球状颗粒并且分散均匀，其平均粒径为2.8nm。FA@Ag NCs的最大激发和发射波长分别为370和447 nm。在最优的检测条件下，FA@Ag NCs可以有效地检测孔雀石绿，线性检测范围为0.5~200 μmol·L-1，检出限为0.084 μmol·L-1，荧光猝灭机理为静态猝灭。基于FA@Ag NCs建立的检测系统已成功用于实际样品中孔雀石绿的检测，并表现出良好的准确性和重现性。 | |
| 关键词: 荧光探针 银纳米团簇 叶酸 孔雀石绿 静态猝灭 | |
| 基金项目: 国家自然科学基金(No.22108193)、山西自然科学应用基础研究项目(No.20210302124210)和大学生创新创业训练项目(No.CXCY2438)资助。 | |
| Abstract: Herein, a one-pot chemical reduction method was reported to prepare folic acid (FA)-stabilized silver nanoclusters (FA@Ag NCs), in which FA, hydrazine hydrate, and silver nitrate were used as capping agent, reducing agent, and precursor, respectively. Several technologies were employed to investigate the structures and optical properties of FA@Ag NCs, including transmission electron microscopy (TEM), X ray photoelectron spectrometer (XPS), Fourier transform infrared spectrometer (FTIR), X-ray diffractometer (XRD), fluorescence spectrometer, and ultraviolet visible absorption spectrometer. FA@Ag NCs were suggested to be highly dispersed and spherical with a size of around 2.8 nm. Moreover, the maximum excitation and emission wavelengths of FA@Ag NCs were 370 and 447 nm, respectively. Under the optimal detection conditions, FA@Ag NCs could be used to effectively detect malachite green with the linear detection range of 0.5-200 μmol·L-1. The detection limit was 0.084 μmol·L-1. The fluorescence-quenching mechanism was ascribed to the static quenching. The detection system based on FA@Ag NCs was successfully used for the detection of malachite green in actual samples with good accuracy and reproducibility. | |
| Keywords: fluorescence probe silver nanoclusters folic acid malachite green static quenching | |
| 投稿时间：2024-11-02 修订日期：2025-03-30 | |
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