| 基于偶氮结构的荧光探针对缺氧肿瘤细胞检测 |
| An azo-based fluorescent probe for the detection of hypoxic tumor cells |
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| 摘要: 肿瘤异常的代谢活动使耗氧量增加，并且通常伴随着血液灌注不良，导致肿瘤区缺氧这一重要特征。选择荧光稳定性较好的大共轭平面荧光团与N，N-二甲基苯胺通过偶氮键共价连接，得到一种对缺氧环境选择性响应的荧光探针AQD。偶氮键的引入使得探针的荧光发射发生显著猝灭，并且探针的偶氮键在缺氧条件下可以被还原断键，其荧光发射逐渐恢复。探针AQD对缺氧环境具有显著的荧光响应，其选择性高、生物相容性好，可用于缺氧A549细胞荧光成像，实现对缺氧肿瘤细胞的检测。 |
| 关键词: 缺氧响应|偶氮化合物|荧光探针|荧光检测|细胞成像 |
| 基金项目: 国家自然科学基金(No.21977015)和中央高校基本科研经费(DUT23YG208)资助。 |
| Abstract: The abnormal metabolic activity of the tumor can increase the oxygen consumption in tumor cells, and the poor blood perfusion often happens in tumor regions as well, which are the main reasons that result in a hypoxic situation in the tumor. A fluorescence probe, AQD, with selective response toward hypoxia was designed for the detection of hypoxic tumor cells, which was obtained by the covalent connection of a large planar conjugated fluorophore with good fluorescence stability and a N,N-dimethylaniline moiety via the azo bond. The introduction of the azo bond in AQD caused significant fluorescence emission quenching, and the probe was reduced under hypoxic conditions to release the fluorophore via breaking the azo bond, resulting in the gradual recovery of fluorescence emission. Probe AQD exhibited a remarkable fluorescence response in hypoxic conditions, high selectivity, and good biocompatibility, which was successfully used for the imaging of hypoxic tumor cells and realized the detection of hypoxic A549 cells. |
| Keywords: hypoxia response|azo compound|fluorescent probe|fluorescence detection|cell imaging |
| 投稿时间：2025-03-12 修订日期：2025-04-03 |
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