| 一种自校准荧光探针用于次氯酸的选择性检测及生物成像 | |
| A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO | |
| 摘要: 我们设计并合成了一种基于亚甲蓝和香豆素双荧光团策略的自校准近红外荧光探针。该探针巧妙地采用亚甲蓝(发射光谱范围：640~740 nm)和香豆素荧光团(发射光谱范围：440~600 nm)作为信号输出单元，实现光谱空间的有效分离，从而对次氯酸(HClO)进行高选择性检测。在生理pH条件下，通过HClO特异性诱导的氧化裂解机制，该探针同步释放出具有红/绿双色荧光发射特性的亚甲蓝和香豆素分子。该探针可对HClO进行快速检测，双通道检出限分别达到25.13 nmol·L-1(绿色通道)和31.55 nmol·L-1(红色通道)。此外，在细胞成像实验中，该探针展现出了良好的细胞膜通透性和低细胞毒性，成功实现了对活细胞内源性和外源性HClO的监测。基于双通道的自校准系统，该探针能够有效减少因光源不稳定或背景干扰等因素导致的信号偏差，显著提高检测的灵敏度和可靠性。 | |
| 关键词: 荧光探针|荧光串扰|自校准|次氯酸|双通道 | |
| 基金项目: 山西省自然科学基金(No.20210302123079)、山西省高校科技创新计划项目(No.2023L334，2020L0616)、山西省科技创新团队(No.202204051001034)长治学院校本应用教材建设项目(XN0329)和山西省1331工程重点创新研究团队资助。 | |
| Abstract: In this study, a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin. The probe utilized methylene blue (emission spectrum range: 640-740 nm) and coumarin fluorophore (emission spectrum range: 440-600 nm) as signal output units, thereby achieving effective spectral separation and highly selective detection of HClO. Under physiological pH conditions, HClO triggers an oxidation-cleavage reaction, releasing methylene blue and coumarin, which emit distinct red and green fluorescence, respectively. This dualemission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L-1 (green channel) and 31.55 nmol·L-1 (red channel). Furthermore, in cell imaging experiments, this probe demonstrated excellent cell membrane permeability and low cytotoxicity, successfully enabling the monitoring of both endogenous and exogenous HClO in living cells. By incorporating a twochannel self-calibration system, the probe effectively mitigated signal variations caused by instrumental or environmental interference, substantially improving detection sensitivity and reliability. | |
| Keywords: fluorescent probe|fluorescent crosstalk|self-calibrated|hypochloric acid|dual channel | |
| 投稿时间：2025-04-02 修订日期：2025-05-09 | |
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