| 双配体修饰铜纳米簇的合成及其奥硝唑检测应用 | |
| Dual-ligand-modified copper nanoclusters: Synthesis and application in ornidazole detection | |
| 摘要: 通过一锅法合成了牛血清白蛋白(BSA)和甘氨酸(Gly)双配体修饰的高荧光强度铜纳米簇(BSA-Gly CuNCs)。基于奥硝唑(ONZ)对BSA-Gly CuNCs的竞争性荧光猝灭和动态猝灭效应，成功建立了一种简便、灵敏的ONZ检测方法。实验结果表明，小分子 Gly 的加入能够更有效地保护 CuNCs，从而增强其荧光强度和稳定性。这种双配体修饰法得到的 BSA-Gly CuNCs 可在0.28~52.60 μmol·L-1的线性范围内检测ONZ，检测限为0.069 μmol·L-1。与单配体修饰的BSA CuNCs相比，双配体修饰的BSA-Gly CuNCs展现出更高的荧光强度、稳定性和传感能力。此外，该方法已成功用于评估实际药品中ONZ的含量。 | |
| 关键词: 铜纳米团簇|奥硝唑|双配体修饰|荧光检测 | |
| 基金项目: 延安市重点产业链项目(No.2024-CYL-068)和陕西省大学生创新训练项目(No.D2023081)资助。 | |
| Abstract: Bovine serum albumin (BSA) and glycine (Gly) dual ligand modified copper nanoclusters (BSA Gly CuNCs) with high fluorescence intensity were synthesized by a one-pot strategy. Based on the competitive fluorescence quenching and dynamic quenching effects of ornidazole (ONZ) on BSA-Gly CuNCs, a simple and sensitive detection method for ONZ was successfully developed. The experimental results demonstrate that the addition of the small molecule Gly can more effectively protect CuNCs, and thus enhance its fluorescence intensity and stability. The proposed assay allowed for the detection of ONZ in a linear range of 0.28 to 52.60 μmol·L-1 and a detection limit of 0.069 μmol·L-1. Compared with the single-ligand-modified CuNCs, dual-ligand-modified BSA-Gly CuNCs had higher fluorescence intensity, stability, and sensing ability and were successfully applied to evaluate ONZ in actual ONZ tablets. | |
| Keywords: copper nanocluster|ornidazole|dual-ligand-modified|fluorescence detection | |
| 投稿时间：2024-10-30 修订日期：2025-04-09 | |
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