| 一例成像活细胞内过氧亚硝酸根阴离子的荧光-比色双模式探针 |
| A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells |
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| 摘要: 本研究开发了一例近红外荧光探针NSHD，该探针由商业原料经一步合成得到。NSHD以荧光-比色双模式快速响应过氧亚硝酸根阴离子(ONOO-)，不受生命体其他常见金属离子、活性物种和生理范围pH变化的干扰。在0~20 μmol·L-1范围内，探针荧光强度与 ONOO-浓度间存在良好的线性关系。质谱测试数据表明 ONOO-使探针发生氧化裂解，导致荧光发射和颜色变化。此外，该探针具有良好的生物相容性，在活HeLa细胞(人类宫颈癌细胞)中实现了对胞内ONOO-的成像。 |
| 关键词: 荧光探针|过氧亚硝酸根阴离子|细胞成像 |
| 基金项目: 国家自然科学基金(No.22107054)、南通市科技计划项目(No.JC2021114)、南通大学大型仪器设备开放基金和南通大学大学生创新训练计划项目资助。 |
| Abstract: Here, we report a near-infrared fluorescent probe, NSHD, which can be easily obtained through a onestep synthesis from a commercial dye. It quickly responded to peroxynitrite anion (ONOO-) both in fluorescence and in colorimetric manners, with negligible interference from other common biological relative species or pH variations under physiological conditions. A good linear relationship was observed between emission intensity and concentration of ONOO- in a range of 0-20 μmol·L-1. The results of MS tests indicated that oxidative cleavages occurred upon ONOO- exposure, resulting in changes to probe emission and color. NSHD successfully imaged the fluctuations of ONOO- in living HeLa cells (human cervical cancer cells), treated by SIN 1 (3 morpholinosydnonimine) or coincubated by LPS (lipopolysaccharide) and IFN-γ (interferon-γ). |
| Keywords: fluorescent probe|peroxynitrite anion|cell imaging |
| 投稿时间：2024-12-02 修订日期：2025-03-20 |
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