| 基于可视化检测肼的吡唑类荧光猝灭型探针 |
| A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine |
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| 摘要: 以吡唑衍生物为荧光基团，合成和表征了一种用于可视化检测N2H4的荧光猝灭型探针(THI)。通过研究其光谱性质发现，THI对N2H4表现出高选择性和高灵敏度，抗干扰能力强，响应时间短(18 s)，它对N2H4的检测限为0.141 μmol·L-1。质谱和密度泛函理论计算验证了N2H4通过THI的分子内电荷转移(ICT)导致荧光猝灭的机理。此外，THI可以被制作成试纸条定量检测N2H4，并且成功实现对HeLa细胞中N2H4的荧光成像。 |
| 关键词: 肼 吡唑衍生物 分子内电荷转移 可视化检测 荧光探针 |
| 基金项目: |
| Abstract: A fluorescence quenching probe (THI) for visually detecting hydrazine (N2H4) was synthesized and characterized by Knoevenagel condensation reaction using pyrazole derivatives as fluorescent groups. Spectroscopic studies revealed that, under optimal excitation and emission wavelengths, the THI exhibited high selectivity and sensitivity toward N2H4, maintaining excellent anti-interference performance even in the presence of various analytes. Additionally, the THI demonstrated ultrafast response time (18 s), attributed to the efficient nucleophilic addition reaction between its optimized molecular recognition site and N2H4. The detection limit for N2H4 was determined to be 0.141 μmol·L-1, meeting the requirements for trace-level analysis. The detection mechanism, verified by mass spectrometry and density functional theory calculations, is ascribed to N2H4-induced intramolecular charge transfer (ICT), leading to fluorescence quenching. Furthermore, THI was successfully fabricated into test strips for the quantitative detection of N2H4 and applied for fluorescence imaging of N2H4 in HeLa cells. |
| Keywords: hydrazine pyrazole derivative intramolecular charge transfer visual detection fluorescent probe |
| 投稿时间：2025-02-25 修订日期：2025-04-24 |
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