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Synthesis and Characterization of Copper(Ⅱ) Complexes with Aroylhydrazone Ligands Containing Quaternary Ammonium Salts: in Vitro DNA Binding and Nuclease Activity
Author NameAffiliationE-mail
Eda Şengül Elif Department of Chemistry, Faculty of Science, Mugla Sı
tkı
Koç
man University, 48000, Kotekli-Mugla, Turkey 
 
Cansu Gökçe Topkaya Department of Chemistry, Faculty of Science, Mugla Sı
tkı
Koç
man University, 48000, Kotekli-Mugla, Turkey 
 
Tolga Göktürk Department of Chemistry, Faculty of Science, Mugla Sı
tkı
Koç
man University, 48000, Kotekli-Mugla, Turkey 
 
Ramazan Gup Department of Chemistry, Faculty of Science, Mugla Sı
tkı
Koç
man University, 48000, Kotekli-Mugla, Turkey 
rgup@mu.edu.tr 
Abstract: Two new N,N,N-trimethylpropane-1-ammonium and N,N,N-trimethylpentane-1-ammonium conjugated acetophenone-(4-hydroxybenzohydrazone) ligands, (E)-3-(4-(1-(2-(4-hydroxybenzoyl)hydrazono)ethyl)phenoxy)-N,N, N-trimethylpropan-1-ammonium perchlorate (H2L1) and (E)-3-(4-(1-(2-(4-hydroxybenzoyl)hydrazono)ethyl)phenoxy)N,N,N-trimethylpentane-1-ammonium perchlorate (H2L2), and two new copper complexes with the formulation[Cu (HL1)2] and[Cu(HL2)2] were synthesized and their DNA binding and cleavage activities of the compounds were investigated. The mechanism of DNA cleavage and the effect of the compound concentration on the DNA cleavage reaction were also studied. UV-Vis spectroscopy results suggest that all compounds preferably bind to DNA via major groove binding mode. This is also supported that DNA cleavage activity was inhibited in the presence of methyl green, a major groove binder. According to the results of electrophoresis studies, the compounds exhibit significant cleavage activity on the plasmid DNA both in the presence and absence of an oxidative agent (H2O2), which strongly depends on the concentration of the compound. The compounds cleavage pBR322 DNA via hydrolytic pathway, which is also supported by the DNA cleavage experiments in the presence of different radical scavengers.
Keywords: hydrazone  copper(Ⅱ) complex  CT-DNA binding  pBR322 DNA cleavage
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Eda Şengül Elif,Cansu Gökçe Topkaya,Tolga Göktürk,Ramazan Gup.Synthesis and Characterization of Copper(Ⅱ) Complexes with Aroylhydrazone Ligands Containing Quaternary Ammonium Salts: in Vitro DNA Binding and Nuclease Activity[J].Chinese Journal of Inorganic Chemistry,2020,36(7):1333-1343.
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Chinese Journal of Inorganic Chemistry