|
| Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes |
| Author Name | Affiliation | E-mail | | WANG Zhen | College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China | | | LI Si-Yuan | School of Materials and Mechanical and Electrical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 China | | | WANG Yuan | College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China | | | WU Wei-Na | College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China | | | ZHANG Lei | College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China | leizh2008@163.com | | CHEN Zhong | School of Materials and Mechanical and Electrical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 China | chenzhonglzu@hotmail.com | | YAN Ling-Ling | School of Physics and Electronic Information Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China | |
|
| Abstract: A novel pyrrole-based hydrazone has been synthesized for colorimetric and fluorescent turn-on detection of Hg2+ ions. A 1:1 binding ratio of the Hg2+ complex has been obtained from Job's plots and MS data. The coordination mode was systematically investigated by 1H NMR, time-resolved fluorescence spectroscopy, and density functional theory (DFT) calculations. The limit of detection of 1 for Hg2+ was as low as 45 nmol·L-1 with an association constant of 5.78×108 L·mol-1. It is worth noting that the response of 1 to Hg2+ was good in a pH range of 4.0 to 10.0. In addition, owing to the existence of the morpholine group, the probe can detect lysosomal Hg2+ in HeLa cells. |
| Keywords: cell imaging fluorescent probe Hg2+ hydrazone lysosome pyrrole |
| Hits: 2850 |
| Download times: 1076 |
| WANG Zhen,LI Si-Yuan,WANG Yuan,WU Wei-Na,ZHANG Lei,CHEN Zhong,YAN Ling-Ling.Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes[J].Chinese Journal of Inorganic Chemistry,2023,39(6):1122-1130. |
| View Full Text View/Add Comment Download reader |
|
|
|
|
|
|
|
|
