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| Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay |
| Author Name | Affiliation | E-mail | | HONG Zheng-Dao | Department of Chemistry, Fudan University, Shanghai 200438, China | | | LI Zhen-Hua | Department of Chemistry, Fudan University, Shanghai 200438, China | | | XU Ming | Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China | | | FENG Wei | Department of Chemistry, Fudan University, Shanghai 200438, China | | | LI Fu-You | Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China | fyli@fudan.edu.cn |
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| Abstract: The functionalized polymer microspheres were prepared by soap-free emulsion polymerization using potassium persulfate (KPS) as the initiator, glycidyl methacrylate (GMA) and styrene (St) as monomers. It was found that microspheres prepared by adding a trace amount of NaCl could be swelled by tetrahydrofuran (THF) to obtain monodisperse hollow microspheres with complete surfaces. The Eu（Ⅲ） complex dye was then loaded into the microspheres shell by means of organic swelling, and immunochromatographic detection of α1 microglobulin (α1-MG) and β2 microglobulin (β2-MG) was performed. |
| Keywords: Eu（Ⅲ） complex glycidyl methacrylate styrene hollow microspheres lateral flow immunoassay |
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| HONG Zheng-Dao,LI Zhen-Hua,XU Ming,FENG Wei,LI Fu-You.Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay[J].Chinese Journal of Inorganic Chemistry,2023,39(11):2055-2064. |
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