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| A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells |
| Author Name | Affiliation | E-mail | | LIU Yanxi | School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China | | | XU Mengjia | School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China | | | CHEN Haonan | School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China | | | LIU Quan | School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China
Xinglin College, Nantong University, Nantong, Jiangsu 226008, China | liu.q@ntu.edu.cn | | ZHANG Yuming | School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China | zym81@ntu.edu.cn |
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| Abstract: Here, we report a near-infrared fluorescent probe, NSHD, which can be easily obtained through a one-step synthesis from a commercial dye. It quickly responded to peroxynitrite anion (ONOO-) both in fluorescence and in colorimetric manners, with negligible interference from other common biological relative species or pH variations under physiological conditions. A good linear relationship was observed between emission intensity and concentration of ONOO- in a range of 0-20 μmol·L-1. The results of MS tests indicated that oxidative cleavages occurred upon ONOO- exposure, resulting in changes to probe emission and color. NSHD successfully imaged the fluctuations of ONOO- in living HeLa cells (human cervical cancer cells), treated by SIN-1 (3-morpholinosydnonimine) or co-incubated by LPS (lipopolysaccharide) and IFN-γ (interferon-γ). |
| Keywords: fluorescent probe peroxynitrite anion cell imaging |
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| LIU Yanxi,XU Mengjia,CHEN Haonan,LIU Quan,ZHANG Yuming.A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells[J].Chinese Journal of Inorganic Chemistry,2025,41(6):1112-1122. |
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