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| A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO |
| Author Name | Affiliation | E-mail | | GAO Wei | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | | | SONG Meiqi | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | | | REN Xuan | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | | | BAI Jianliang | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | | | SU Jing | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | | | MA Jianlong | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | majianlong2021@163.com | | WANG Zhijun | Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China | wzhijun@sxu.edu.cn |
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| Abstract: In this study, a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin. The probe utilized methylene blue (emission spectrum range: 640-740 nm) and coumarin fluorophore (emission spectrum range: 440-600 nm) as signal output units, thereby achieving effective spectral separation and highly selective detection of HClO. Under physiological pH conditions, HClO triggers an oxidation-cleavage reaction, releasing methylene blue and coumarin, which emit distinct red and green fluorescence, respectively. This dualemission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L-1 (green channel) and 31.55 nmol·L-1 (red channel). Furthermore, in cell imaging experiments, this probe demonstrated excellent cell membrane permeability and low cytotoxicity, successfully enabling the monitoring of both endogenous and exogenous HClO in living cells. By incorporating a two-channel self-calibration system, the probe effectively mitigated signal variations caused by instrumental or environmental interference, substantially improving detection sensitivity and reliability. |
| Keywords: fluorescent probe fluorescent crosstalk self-calibrated hypochloric acid dual channel |
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| GAO Wei,SONG Meiqi,REN Xuan,BAI Jianliang,SU Jing,MA Jianlong,WANG Zhijun.A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO[J].Chinese Journal of Inorganic Chemistry,2025,41(6):1173-1182. |
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