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| A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine |
| Author Name | Affiliation | E-mail | | ZHANG Yingpeng | School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China | yingpengzhang@126.com | | LI Xingxing | School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China | | | YANG Yunshang | School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China | | | TENG Zhidong | Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China | |
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| Abstract: A fluorescence quenching probe (THI) for visually detecting hydrazine (N2H4) was synthesized and characterized by Knoevenagel condensation reaction using pyrazole derivatives as fluorescent groups. Spectroscopic studies revealed that, under optimal excitation and emission wavelengths, the THI exhibited high selectivity and sensitivity toward N2H4, maintaining excellent anti-interference performance even in the presence of various analytes. Additionally, the THI demonstrated ultrafast response time (18 s), attributed to the efficient nucleophilic addition reaction between its optimized molecular recognition site and N2H4. The detection limit for N2H4 was determined to be 0.141 μmol·L-1, meeting the requirements for trace-level analysis. The detection mechanism, verified by mass spectrometry and density functional theory calculations, is ascribed to N2H4-induced intramolecular charge transfer (ICT), leading to fluorescence quenching. Furthermore, THI was successfully fabricated into test strips for the quantitative detection of N2H4 and applied for fluorescence imaging of N2H4 in HeLa cells. |
| Keywords: hydrazine pyrazole derivative intramolecular charge transfer visual detection fluorescent probe |
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| ZHANG Yingpeng,LI Xingxing,YANG Yunshang,TENG Zhidong.A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine[J].Chinese Journal of Inorganic Chemistry,2025,41(7):1301-1308. |
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